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Anti-Mouse CD3e Functional Grade Purified

Also known as: CD3 epsilon

RUO: For Research Use Only
anti-mouse CD3 antibody: no azide, sterile, azide free anti-CD3e 145-2c11 mAbanti-mouse CD3 antibody: no azide, sterile, azide free anti-CD3e 145-2c11 mAbStaining of mouse splenocytes with Anti-Mouse CD3e FITC (left) or PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse CD3e Functional Grade Purified
Catalog Number: 16-0031
Concentration: 1 mg/ml
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 2-8°C.
Handling Conditions: Use in sterile envrioment.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 145-2C11
Host/Isotype: Armenian Hamster IgG
 
Product Options
Cat. No. Size Price Add Qty to Cart
16-0031-81 50 ug
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16-0031-82 100 ug
16-0031-85 500 ug
16-0031-86 1 mg
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
 
Research Related Products
Cat. No.Format
11-4111 Anti-Armenian Hamster IgG FITC
11-4317 Streptavidin FITC
12-4317 Streptavidin PE
13-4113 Anti-Armenian Hamster IgG Biotin (Polyclonal)
16-4888 Armenian Hamster IgG Isotype Control Functional Grade Purified (eBio299Arm)
17-4317 Streptavidin APC
 

Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
11-0031 Anti-Mouse CD3e FITC 488 518 FC RUO
12-0031 Anti-Mouse CD3e PE 488 575 FC RUO
13-0031 Anti-Mouse CD3e Biotin N/A N/A FC RUO
14-0031 Anti-Mouse CD3e Purified N/A N/A FA FC IHC (F) IP WB RUO
15-0031 Anti-Mouse CD3e PE-Cy5 488 670 FC RUO
16-0031 Anti-Mouse CD3e Functional Grade* Purified N/A N/A FA FC RUO
17-0031 Anti-Mouse CD3e APC 633 660 FC RUO
25-0031 Anti-Mouse CD3e PE-Cy7 488 760 FC RUO
36-0031 Anti-Mouse CD3e Functional Grade* Biotin N/A N/A FA FC IHC (F) IP WB RUO
45-0031 Anti-Mouse CD3e PerCP-Cy5.5 488 690 FC RUO
53-0031 Anti-Mouse CD3e Alexa Fluor® 488 488 519 FC RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
 
Description
The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 145-2C11 antibody has been reported for use in flow cytometric analysis. It has also been reported in cell activation and cell depletion. Please visit the following website to view a protocol for in vitro T-cell activation:
http://www.ebioscience.com/ebioscience/appls/AC145.htm
Applications Tested
The 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocyte and thymocyte suspensions. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Geng H, Zhang GM, Xiao H, Yuan Y, Li D, Zhang H, Qiu H, He YF, Feng ZH. HSP70 vaccine in combination with gene therapy with plasmid DNA encoding sPD-1 overcomes immune resistance and suppresses the progression of pulmonary metastatic melanoma. Int J Cancer. 2006 Jun 1;118(11):2657-64. (145-2C11, IHC frozen, PubMed)

Krieg C, Han P, Stone R, Goularte OD, Kaye J. Functional analysis of B and T lymphocyte attenuator engagement on CD4+ and CD8+ T cells. J Immunol. 2005 Nov 15;175(10):6420-7. (145-2C11, FA, PubMed)

Takeuchi A, Usui Y, Takeuchi M, Hattori T, Kezuka T, Suzuki J, Okunuki Y, Iwasaki T, Haino M, Matsushima K, Usui M. CCR5-deficient mice develop experimental autoimmune uveoretinitis in the context of a deviant effector response. Invest Ophthalmol Vis Sci. 2005 Oct;46(10):3753-60. (145-2C11, IHC frozen, PubMed)

Schuchert MJ, Wright RD, Colson YL. Characterization of a newly discovered T-cell receptor beta-chain heterodimer expressed on a CD8+ bone marrow subpopulation that promotes allogeneic stem cell engraftment. Nat Med. 2000 Aug;6(8):904-9. (145-2C11, WB and IP)

Kearse KP. Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes. J Biol Chem. 1998 Jun 5;273(23):14152-7. (145-2C11, IP and WB)

Samelson LE, O'Shea JJ, Luong H, Ross P, Urdahl KB, Klausner RD, Bluestone J. T cell antigen receptor phosphorylation induced by an anti-receptor antibody. J Immunol. 1987 Oct 15;139(8):2708-14.

Leo O, Foo M, Sachs DH, Samelson LE, Bluestone JA. Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proc Natl Acad Sci U S A. 1987 Mar;84(5):1374-8.


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