Functional Grade Purified anti-mouse CD3e

RUO: For Research Use Only

anti-mouse CD3 antibody: no azide, sterile, azide free anti-CD3e 145-2c11 mAb

Surface staining of mouse splenocytes with anti-mouse CD3e (145-2C11) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Contents: Functional Grade Purified anti-mouse CD3e
Catalog Number: 16-0031
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Clone: 145-2C11
Host/Isotype: Armenian Hamster IgG
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
16-0031-81	50 ug	To view relevant pricing, please select your country and resume browsing.
16-0031-82	100 ug
16-0031-85	500 ug
16-0031-86	1 mg

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-4111	FITC Anti-Armenian Hamster IgG
11-4317	Streptavidin-FITC (Fluorescein isothiocyanate)
12-4317	Streptavidin-PE (Phycoerythrin)
13-4113	Biotin Anti-Armenian Hamster IgG (Polyclonal)
16-4444	Functional Grade Purified Armenian Hamster IgG Isotype Control (n/a)
16-4888	Functional Grade Purified Armenian Hamster IgG Isotype Control (eBio299Arm)
17-4317	Streptavidin Allophycocyanin (SA-APC)

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-0031	FITC Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	518	FC	RUO
12-0031	PE Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	575	FC	RUO
13-0031	Biotin Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FC	RUO
14-0031	Affinity Purified Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC IH/F IP WB	RUO
15-0031	PE-Cy5 Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	670	FC	RUO
16-0031	Functional Grade* Purified Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC	RUO
17-0031	APC Hamster anti-mouse CD3 (epsilon subunit; CD3e)	633	660	FC	RUO
25-0031	PE-Cy7 Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	760	FC	RUO
36-0031	Functional Grade* Biotin anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC IH/F IP WB	RUO
45-0031	PerCP-Cy5.5 anti-mouse CD3 (epsilon subunit; CD3e) (PE-Cy5.5 replacement)	488	690	FC	RUO
53-0031	Alexa Fluor® 488 anti-mouse CD3 (epsilon subunit; CD3e)	488	519	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 145-2C11 antibody has been reported for use in flow cytometric analysis. It has also been reported in cell activation and cell depletion. Please visit the following website to view a protocol for in vitro T-cell activation:
http://www.ebioscience.com/ebioscience/appls/AC145.htm

Applications Tested

The 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocyte and thymocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Geng H, Zhang GM, Xiao H, Yuan Y, Li D, Zhang H, Qiu H, He YF, Feng ZH. HSP70 vaccine in combination with gene therapy with plasmid DNA encoding sPD-1 overcomes immune resistance and suppresses the progression of pulmonary metastatic melanoma. Int J Cancer. 2006 Jun 1;118(11):2657-64. (145-2C11, IHC frozen, PubMed)

Krieg C, Han P, Stone R, Goularte OD, Kaye J. Functional analysis of B and T lymphocyte attenuator engagement on CD4+ and CD8+ T cells. J Immunol. 2005 Nov 15;175(10):6420-7. (145-2C11, FA, PubMed)

Takeuchi A, Usui Y, Takeuchi M, Hattori T, Kezuka T, Suzuki J, Okunuki Y, Iwasaki T, Haino M, Matsushima K, Usui M. CCR5-deficient mice develop experimental autoimmune uveoretinitis in the context of a deviant effector response. Invest Ophthalmol Vis Sci. 2005 Oct;46(10):3753-60. (145-2C11, IHC frozen, PubMed)

Schuchert MJ, Wright RD, Colson YL. Characterization of a newly discovered T-cell receptor beta-chain heterodimer expressed on a CD8+ bone marrow subpopulation that promotes allogeneic stem cell engraftment. Nat Med. 2000 Aug;6(8):904-9. (145-2C11, WB and IP)

Kearse KP. Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes. J Biol Chem. 1998 Jun 5;273(23):14152-7. (145-2C11, IP and WB)

Samelson LE, O'Shea JJ, Luong H, Ross P, Urdahl KB, Klausner RD, Bluestone J. T cell antigen receptor phosphorylation induced by an anti-receptor antibody. J Immunol. 1987 Oct 15;139(8):2708-14.

Leo O, Foo M, Sachs DH, Samelson LE, Bluestone JA. Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proc Natl Acad Sci U S A. 1987 Mar;84(5):1374-8.