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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells. Applications ReportedFor research use only, not for diagnostic or therapeutic use. The 145-2C11 antibody has been reported for use in flow cytometric analysis. It has also been reported in cell activation and cell depletion. Please visit the following website to view a protocol for in vitro T-cell activation: http://www.ebioscience.com/ebioscience/appls/AC145.htm Applications TestedThe 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocyte and thymocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. References
Leo, O., M. Foo, et al. (1987). "Identification of a monoclonal antibody specific for a murine T3 polypeptide." Proc Natl Acad Sci U S A 84(5): 1374-8. Samelson, L. E., J. J. O'Shea, et al. (1987). "T cell antigen receptor phosphorylation induced by an anti-receptor antibody." J Immunol 139(8): 2708-14. Related ProductsCat. 11-4111 FITC Anti-Armenian Hamster IgG Cat. 13-4113 Biotin Anti-Armenian Hamster IgG (clone Polyclonal) Cat. 11-4317 Streptavidin-FITC (Fluorescein isothiocyanate) Cat. 12-4317 Streptavidin-PE (Phycoerythrin) Cat. 17-4317 Streptavidin Allophycocyanin (SA-APC) Cat. 16-4444 Functional Grade Purified Armenian Hamster IgG Isotype Control (clone n/a) Cat. 16-4888 Functional Grade Purified Armenian Hamster IgG Isotype Control (clone eBio299Arm) |
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