Product Information

Contents: Functional Grade Purified anti-human CD2 (LFA-2)
Catalog Number: 16-0029
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: RPA-2.10
Isotype: Mouse IgG1, κ
HLDA No.: IV T085

Surface staining of normal human peripheral blood cells with anti-human CD2 (RPA-2.10) FITC (left), and PE (right). Autofluorescence is indicated by open histogram. Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0029	Affinity Purified anti-human CD2 (LFA-2)	N/A	N/A	FA FC IH/F WB
16-0029	Functional Grade* Purified anti-human CD2 (LFA-2)	N/A	N/A	FC
13-0029	Biotin anti-human CD2 (LFA-2)	N/A	N/A	FC
11-0029	FITC anti-human CD2 (LFA-2)	488	518	FC
12-0029	PE anti-human CD2 (LFA-2)	488	575	FC
15-0029	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD2 (LFA-2)	488	670	FC
17-0029	Allophycocyanin (APC) anti-human CD2 (LFA-2)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RPA-2.10 antibody been reported for use in flow cytometric analysis. RPA-2.10 has also been reported in blocking of CD2 activity in in vitro functional assays

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Aversa GG, Bishop GA, Suranyi MG, Hall BM. (1987). RPA-2.10: an anti-CD2 monoclonal antibody that inhibits alloimmune responses and monitors T cell activation. Transplant Proc. 19:277-8

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