Product Information

Contents: Functional Grade Purified anti-mouse CD2 (LFA-2)
Catalog Number: 16-0021
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: RM2-5
Isotype: Rat IgG2b, λ

Staining of C57Bl/6 splenocytes with 0.125 μg of FG Purified Rat IgG2b Iso Cntrl (cat. 16-4331) (open histogram) or 0.125 μg of FG Purified anti-mouse CD2 (RM2-5) (colored histogram) followed by FITC anti-rat IgG (cat. 11-4811). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0021	Affinity Purified anti-mouse CD2 (LFA-2)	N/A	N/A	FA FC IH/F
16-0021	Functional Grade* Purified anti-mouse CD2 (LFA-2)	N/A	N/A	FA FC
13-0021	Biotin anti-mouse CD2 (LFA-2)	N/A	N/A	FC
11-0021	FITC anti-mouse CD2 (LFA-2)	488	518	FC
12-0021	PE anti-mouse CD2 (LFA-2)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The RM2-5 monoclonal antibody reacts with the mouse CD2 molecule, an approximately 50-55 kDa cell surface receptor expressed by all mouse lymphocytes. Expression of the CD2 antigen in the mouse differs from that of the human in that B lineage cells in the mouse from the pre-B cell stage to mature B cells express CD2. CD2 is a ligand for CD48 in the mouse and is involved in adhesion and activation of T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RM2-5 antibody has been reported for use in flow cytometric analysis. RM2-5 has also been reported in in vitro blocking of mouse CD2 (LFA-2)

Applications Tested

The RM2-5 antibody has been tested by flow cytometric analysis of mouse splenocyte and bone marrow cell suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nakamura, T., K. Takahashi, et al. (1990). "Relative contribution of CD2 and LFA-1 to murine T and natural killer cell functions." J Immunol 145(11): 3628-34.
Yagita, H., T. Nakamura, et al. (1989). "CD2 expression in murine B cell lineage." Int Immunol 1(1): 94-8.
Yagita, H., T. Nakamura, et al. (1989). "Monoclonal antibodies specific for murine CD2 reveal its presence on B as well as T cells." Proc Natl Acad Sci U S A 86(2): 645-9.

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Cat. 11-4811  FITC Anti-Rat IgG
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