Functional Grade anti-mouse CD1d (CD1d1/CD1d2, CD1.1/CD1.2, Ly-38); azide free, no azide

Contents: Functional Grade Purified anti-mouse CD1d (CD1.1, Ly-38)
Catalog Number: 16-0011
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
KEEP CONTENTS STERILE.
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
Clone: 1B1
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
16-0011-81	50 ug	$80
16-0011-82	100 ug	$150
16-0011-85	500 ug	$280

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes with 1.0 µg of FG purified rat IgG2b isotype control (cat.16-4331) (open histogram), or 1.0 µg of FG purified anti-mouse CD1d (1B1) (colored histogram) followed by FITC anti-rat IgG (cat. 11-4811). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0011	FITC anti-mouse CD1d (CD1.1, Ly-38)	488	518	FC
12-0011	PE anti-mouse CD1d (CD1.1, Ly-38)	488	575	FC
13-0011	Biotin anti-mouse CD1d (CD1.1, Ly-38)	N/A	N/A	FC
14-0011	Affinity Purified anti-mouse CD1d (CD1.1, Ly-38)	N/A	N/A	FA FC IHC IP
16-0011	Functional Grade* Purified anti-mouse CD1d (CD1.1, Ly-38)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein with structural homology to MHC class I molecules. While similar to MHC Class I, CD1d associates with β2-m, functionally CD1d is similar to MHC Class II. 1B1 detects CD1d at varying levels on mouse leukocytes. 1B1 detects β2-m associated CD1d.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 1B1 antibody has been reported for use in flow cytometric analysis. 1B1 has also been reported in in vivo and in vitro studies.

Applications Tested

The 1B1 antibody has been tested by flow cytometeric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Brossay L, D.Jullien, S. Cardell, B.C. Sydora, N. Burdin, R.L. Modlin, and M. Kronenberg. 1997. Mouse CD1 is mainly expressed on hemopoietic derived cells. J. Immunol. 159: 1216-1224.
Amano M., N. Baumgarth, M.D. Dick, L. Brossay, M. Kronenberg, L.A. Herzenberg, and S Strober. 1998. CD1 expression defines subsets of follicular and marginal zone B cells in the spleen: β2-microglobulin-dependent and independent forms. J. Immunol. 161:1710-1717.
Sydora B.C., L. Brossay, A. Hagenbaugh, M. Kronenberg, and H. Cheroutre. 1996. TAP-independent selection of CD8+ intestinal intraepithelial lymphocytes. J. Immunol. 156: 4209-4216.
Roark J.H., S.-H. Park, J. Jayawardena, U. Kavita, M. Shannon, and A. Bendelac. 1998. CD1.1 expression by mouse antigen-presenting cells and marginal zone B cells. J. Immunol. 160: 3121-3127.
Kawano, T. J. Cui, Y. Koezuka, I. Toura, Y. Kaneko, K. Motoki, H. Ueno, R. Nakagawa, H. Sato, E. Kondo, H. Koseki, and M. Taniguchi. 1997. CD1d-restricted and TCR-mediated activation of Valpha14 NKT cells by glycosylceramides. Science 278:1626-1629.
Brudin N., L. Brossay, Y. Koezuka, S. T. Smiley, M. J. Grusby, M. Gui, M. Taniguchi, K. Hayakawa, M. Kronenberg. 1998. Selective Ability of Mouse CD1 to Present Glycolipids: α-Galactosylceramide Specifically Stimulates Vα14+ NK T Lymphocytes. J. Immunol 161: 271-81.
Szalay G, Ladel CH, Blum C, Brossay L, Kronenberg M, Kaufmann SH. Cutting edge: anti-CD1 monoclonal antibody treatment reverses the production patterns of TGF-beta 2 and Th1 cytokines and ameliorates listeriosis in mice. J Immunol. 1999 Jun 15;162(12):6955-8. (in vivo, PubMed)

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