Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-human HLA-ABC
Catalog Number: 15-9983
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: W6/32
Isotype: Mouse IgG2a, κ

Staining of normal human peripheral blood cells with staining buffer(autofluorescence) (open histogram) or PE-Cy5 W6/32 (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9983	Affinity Purified anti-human HLA-ABC	N/A	N/A	FC IH/F IP
16-9983	Functional Grade* Purified anti-human HLA-ABC	N/A	N/A	FC
13-9983	Biotin anti-human HLA-ABC	N/A	N/A	FC
11-9983	FITC anti-human HLA-ABC	488	518	FC
12-9983	PE anti-human HLA-ABC	488	575	FC
15-9983	PE-Cy5 anti-human HLA-ABC	488	670	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The W6/32 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The W6/32 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Barnstable, C. J., W. F. Bodmer, et al. 1978. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell 14(1): 9-20.

Koene G, Arts-Hilkes, et al.2004. High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss. Hum Immunol. 65(12):1455-62. (IHC frozen, PubMed)

Goldman-Wohl D, Ariel I, et al. 2001. A study of human leukocyte antigen G expression in hydatidiform moles. Am J Obstet Gynecol. 185(2):476-80. (IHC frozen, PubMed)

Stern PL, Beresford N, et al. 1987. Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast. J Immunol. 138(4):1088-91. (baboon cross-reactivity, PubMed)

Related Products

Cat. 15-4724    Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG2a, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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