PE-Cy5 anti-human HLA-DR antibody: Cy5PE conjugate

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Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-human HLA-DR
Catalog Number: 15-9956
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: LN3
Isotype: Mouse IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
15-9956-71	25 tests	$120
15-9956-73	100 tests	$260

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG2b Iso Cntrl (cat. 15-4732) (open histogram) or PE-Cy5 anti-human HLA-DR (LN3) (colored histogram). Cells in the lymphocyte (left) and monocyte (right) gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9956	Affinity Purified anti-human HLA-DR	N/A	N/A	FC IHC
13-9956	Biotin anti-human HLA-DR	N/A	N/A	FC
11-9956	Fluorescein isothiocyanate (FITC) anti-human HLA-DR	488	518	FC
12-9956	PE anti-human HLA-DR	488	575	FC
15-9956	Phycoerythrin-Cy5 (PE-Cy5) anti-human HLA-DR	488	670	FC
17-9956	APC anti-human HLA-DR	633	660	FC
25-9956	Phycoerythrin-Cy7 (PE-Cy7) anti-human HLA-DR	488	760	FC
56-9956	Alexa Fluor® 700 anti-human HLA-DR (APC-Cy5.5 Equivalent)	633	723	FC
27-9956	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human HLA-DR	633	779	FC
45-9956	PerCP-Cy5.5 anti-human HLA-DR	488	690	FC
93-9956	eFluor^TM 605^NC anti-human HLA-DR	350-500	605	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of α and β subunits and plays an important role in the presentation of peptides to CD4⁺ T lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This LN3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This LN3 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.015 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Fullen, D. R. and J. T. Headington (1998). "Factor XIIIa-positive dermal dendritic cells and HLA-DR expression in radial versus vertical growth-phase melanomas [see comments]." J Cutan Pathol 25(10): 553-8.
Hua, Z. X., K. E. Tanaka, et al. (1998). "Immunoreactivity for LN2 and LN3 distinguishes small cell carcinomas from non-small cell carcinomas in the lung." Hum Pathol 29(12): 1441-6.
Ioachim, H. L., S. E. Pambuccian, et al. (1996). "Lymphoid monoclonal antibodies reactive with lung tumors. Diagnostic applications." Am J Surg Pathol 20(1): 64-71.
Davey, F. R., S. Olson, et al. (1988). "The immunophenotyping of extramedullary myeloid cell tumors in paraffin-embedded tissue sections." Am J Surg Pathol 12(9): 699-707.
Norton, A. J. and P. G. Isaacson (1987). "Detailed phenotypic analysis of B-cell lymphoma using a panel of antibodies reactive in routinely fixed wax-embedded tissue." Am J Pathol 128(2): 225-40.

Related Products

Cat. 15-4732    Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG2b Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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