PE-Cy5 anti-mouse/rat ICOS (CD278) antibody: Cy5PE conjugate

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Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-mouse/rat ICOS (CD278)
Catalog Number: 15-9949
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: C398.4A
Isotype: Armenian Hamster IgG

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
15-9949-81	50 ug	$160
15-9949-82	100 ug	$260

*International customers: Please contact your distributor for region specific pricing.

Surface staining of ConA stimulated mouse spenocytes with anti-mouse/rat ICOS (C398.4A) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-9949	FITC anti-mouse/rat ICOS (CD278)	488	518	FC
12-9949	PE anti-mouse/rat ICOS (CD278)	488	575	FC
13-9949	Biotin anti-mouse/rat ICOS (CD278)	N/A	N/A	FC
14-9949	Affinity Purified anti-mouse/rat ICOS (CD278)	N/A	N/A	FA FC IHC IP
15-9949	PE-Cy5 anti-mouse/rat ICOS (CD278)	488	670	FC
16-9949	Functional Grade* Purified anti-mouse/rat ICOS (CD278)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The C398.4A mAb reacts with ICOS (Inducible COStimulatory molecule), also known as H4/CRP-1/AILIM, and cross-reacts with mouse and rat ICOS. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. A dimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1, is thought to play a protective role in inflammatory autoimmune diseases and be involved in the development of Th2 cells. C398.4A is reported to display strong costimulation of proliferation of T cell clones, intermediate costimulation on activated T cells, and weak costimulation on fresh resting T cells. It is a good costimulator of IL-10 production especially from pre-activated cells that express high level of H4/ICOS. The epitope recognized by C398.4A is different from that bound by the ICOSL (i.e. C398.4A does not block binding of ICOSL).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The C398.4A antibody has been reported for use in flow cytometric analysis.

Applications Tested

This C398.4A antibody has been tested by flow cytometric analysis of mouse ConA-activated (2 days) spleen cell suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Lucia, M. B., D. Buonfiglio, et al. 2000. Expression of the novel T cell activation molecule hpH4 in HIV-infected patients: correlation with disease status. AIDS Res Hum Retroviruses 16(6): 549-57.
Buonfiglio, D., M. Bragardo, et al. 1999. Characterization of a novel human surface molecule selectively expressed by mature thymocytes, activated T cells and subsets of T cell lymphomas. Eur J Immunol 29(9): 2863-74.
Redoglia, V., U. Dianzani, et al. 1996. Characterization of H4: a mouse T lymphocyte activation molecule functionally associated with the CD3/T cell receptor. Eur J Immunol 26(11): 2781-9.
Arimura Y, Shiroki F, Kuwahara S, Kato H, Dianzani U, Uchiyama T, Yagi J. Akt is a neutral amplifier for Th cell differentiation. J Biol Chem. 2004 Mar 19;279(12):11408-16. Epub 2004 Jan 5. [activating]

Related Products

Cat. 15-4888    Phycoerythrin-Cy5 (PE-Cy5) Armenian Hamster IgG Isotype Control (clone eBio299Arm)
Cat. 12-9940    PE anti-mouse ICOS (CD278) (clone 15F9)
Cat. 13-9940    Biotin anti-mouse ICOS (CD278) (clone 15F9)
Cat. 14-9940    Affinity Purified anti-mouse ICOS (CD278) (clone 15F9)
Cat. 15-9940    PE-Cy5 anti-mouse ICOS (CD278) (clone 15F9)
Cat. 12-9942    PE anti-mouse ICOS (CD278) (clone 7E.17G9)
Cat. 13-9942    Biotin anti-mouse ICOS (CD278) (clone 7E.17G9)
Cat. 14-9942    Affinity Purified anti-mouse ICOS (CD278) (clone 7E.17G9)
Cat. 15-9942    PE-Cy5 anti-mouse ICOS (CD278) (clone 7E.17G9)
Cat. 16-9942    Functional Grade Purified anti-mouse ICOS (CD278) (clone 7E.17G9)
Cat. 11-9948    FITC anti-human ICOS (CD278) (clone ISA-3)
Cat. 12-9948    Phycoerythrin (PE) anti-human ICOS (CD278) (clone ISA-3)
Cat. 13-9948    Biotin anti-human ICOS (CD278) (clone ISA-3)
Cat. 14-9948    Affinity Purified anti-human ICOS (CD278) (clone ISA-3)
Cat. 16-9948    Functional Grade Purified anti-human ICOS (CD278) (clone ISA-3)

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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