Product Information

Contents: Phycoerythrin (PE)-Cy5 anti-mouse ICOS (CD278)
Catalog Number: 15-9940
Sizes: 50 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 15F9
Isotype: Golden Syrian Hamster IgG

Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 μg of PE-Cy5 15F9 (colored histogram). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9940	Affinity Purified anti-mouse ICOS (CD278)	N/A	N/A	FC
13-9940	Biotin anti-mouse ICOS (CD278)	N/A	N/A	FC
12-9940	PE anti-mouse ICOS (CD278)	488	575	FC
15-9940	PE-Cy5 anti-mouse ICOS (CD278)	488	670	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 15F9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 15F9 has been reported for use in flow cytometric analysis.

Applications Tested

The 15F9 antibody has been tested by flow cytometric analysis of mouse ConA-activated splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

McAdam, A. J., T. T. Chang, et al. 2000. Mouse inducible costimulatory molecule (ICOS) expression is enhanced by CD28 costimulation and regulates differentiation of CD4+ T cells. J Immunol 165(9): 5035-40.

Lucy S. K. Walker, Helen E. Wiggett, Fabrina M. C. Gaspal, Chandra R. Raykundalia, Margaret D. Goodall, Kai-Michael Toellner, and Peter J. L. Lane. 2003. Established T Cell-Driven Germinal Center B Cell Proliferation Is Independent of CD28 Signaling but Is Tightly Regulated Through CTLA-4. J Immunol. 170:91-98.

Kohyama M, Sugahara D, Sugiyama S, Yagita H, Okumura K, Hozumi N. Inducible costimulator-dependent IL-10 production by regulatory T cells specific for self-antigen. Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4192-7. Epub 2004 Mar 10. (blocking, PubMed)

Related Products

Cat. 15-4914  PE-Cy5 Golden Syrian Hamster IgG Isotype Control (clone n/a)
Cat. 12-9942  PE anti-mouse ICOS (CD278) (clone 7E.17G9)
Cat. 13-9942  Biotin anti-mouse ICOS (CD278) (clone 7E.17G9)
Cat. 14-9942  Affinity Purified anti-mouse ICOS (CD278) (clone 7E.17G9)
Cat. 15-9942  PE-Cy5 anti-mouse ICOS (CD278) (clone 7E.17G9)
Cat. 16-9942  Functional Grade Purified anti-mouse ICOS (CD278) (clone 7E.17G9)
Cat. 11-9949  FITC anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 12-9949  PE anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 13-9949  Biotin anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 14-9949  Affinity Purified anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 15-9949  PE-Cy5 anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 16-9949  Functional Grade Purified anti-mouse/rat ICOS (CD278) (clone C398.4A)

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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