Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-human CD45RB
Catalog Number: 15-7457
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MEM-55 (MEM55)
Isotype: Mouse IgG1
HLDA No.: III

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7457	Affinity Purified anti-human CD45RB	N/A	N/A	FC
11-7457	Fluorescein isothiocyanate (FITC) anti-human CD45RB	488	518	FC
12-7457	Phycoerythrin (PE) anti-human CD45RB	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MEM-55 monoclonal antibody reacts with human CD45RB, the 190, 205, and 220 kDa isoforms of the leucocyte common antigen (LCA). CD45RB is expressed on a subset of B cells, T cells, monocytes, and at lower density on granulocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MEM-55 (MEM55) antibody has been reported for use in flow cytometric analysis.

Applications Tested

The MEM-55 (MEM55) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

McMichael, A.J., P.C.L. Beverly, et al. eds. 1987. Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 15-4714  Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

1.         Sale, lease, license or other transfer of the material or any material derived or produced from it.
2.         Sale, lease, license or other grant of rights to use this material or any other material derived or produced from it
3.         Use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO eBioscience AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.