Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-mouse DO11.10 TCR
Catalog Number: 15-5808
Sizes: 25 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: KJ1-26 (KJ126, KJI-26, KJI26)
Isotype: Mouse IgG2a

Staining of DO11.10 T cell line with 0.125 μg of PE-Cy5 Rat IgG1 Iso Cntrl (cat. 15-4301) (blue histogram) or 0.125 μg of PE-Cy5 anti-mouse DO11.10 TCR (KJ1-26) (purple histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5808	Affinity Purified anti-mouse DO11.10 TCR	N/A	N/A	FA FC IP
13-5808	Biotin anti-mouse DO11.10 TCR	N/A	N/A	FC
11-5808	Fluorescein isothiocyanate (FITC) anti-mouse DO11.10 TCR	488	518	FC
12-5808	Phycoerythrin (PE) anti-mouse DO11.10 TCR	488	575	FC
15-5808	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse DO11.10 TCR	488	670	FC
17-5808	Allophycocyanin (APC) anti-mouse DO11.10 TCR	633	660	FC
35-5808	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse DO11.10 TCR	488	690	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested.The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-A^d major histocompatibility molecules.The use of DO11.10 and KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been tested by flow cytometric analysis of the DO11.10 T cell hybridoma. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Haskins, K., R. Kubo, J. White, M. Pigeon, J. Kappler, and P. Marrack. 1983. The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody. J. Exp. Med. 157: 1149- 69.

Marrack, P., R. Shimonkevitz, C. Hannum, K. Haskins, and J. Kappler. 1983. The major histocompatibility complex-restricted antigen receptor on T cells. IV. An anti-idiotypic antibody predicts both antigen and Ispecificity. J. Exp. Med. 158: 1635-46.

Murphy, K. M., A. B. Heimberger, and D. Y. Loh. 1990. Induction by antigen of intrathymic apoptosis of CD4+ CD8+ TCR lo thymocytes in vivo. Science. 250: 1720-3.

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Cat. 15-4724  Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG2a, K Isotype Control

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