Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-mouse gamma delta TCR (γδ-TCR, γδTCR)
Catalog Number: 15-5711
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioGL3 (GL-3, GL3)
Isotype: Armenian Hamster IgG

Staining of C57Bl/6 lymph node cells with PE anti-mouse CD3e (145-2C11) (cat. 12-0031) and 0.5 μg of PE-Cy5 Armenian Hamster IgG Iso Cntrl (cat. 15-4888) (left) or 0.5 μg of PE-Cy5 anti-mouse gdTCR (GL3) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5711	Affinity Purified anti-mouse gamma delta TCR (gd-TCR, gdTCR)	N/A	N/A	FC IP
13-5711	Biotin anti-mouse gamma delta TCR (gd-TCR, gdTCR)	N/A	N/A	FC
11-5711	Fluorescein isothiocyanate (FITC) anti-mouse gamma delta TCR (gd-TCR, gdTCR)	488	518	FC
12-5711	Phycoerythrin (PE) anti-mouse gamma delta TCR (gd-TCR, gdTCR)	488	575	FC
15-5711	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse gamma delta TCR (gd-TCR, gdTCR)	488	670	FC
17-5711	Allophycocyanin (APC) anti-mouse gamma delta TCR (gd-TCR, gdTCR)	633	660	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The GL3 monoclonal antibody (mAb) reacts with the γδ T-cell Receptor complex (TCR) on all γδ TCR bearing T cells, but it does not react with aß TCR. The γδ TCR is present on cells in the thymus, epidermis, epithelial lining of the intestine, peritoneal cavity, and lymphoid tissues.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This GL3 (GL-3) antibody has been reported for use in flow cytometric analysis.

It is recommended to prestain cells with anti-mouse CD16/32 (cat. 14-0161) to prevent non-specific Fc-mediated binding of the GL3 monoclonal antibody.

Applications Tested

This eBioGL3 (GL-3, GL3) antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Goodman, T., and L. LeFrançois. 1989. Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function. J. Exp. Med. 170: 1569-1581.

MacDonald, H. R., M. Schreyer, R. C. Howe, and C. Bron. 1990. Selective expression of CD8a (Ly-2) subunit on activated thymic γδ cells. Eur. J. Immunol. 20: 927-930.

LeFrançois, L. 1991. Phenotypic complexity of intraepithelial lymphocytes of the small intestine. J. Immunol. 147: 1746-1751.

Related Products

Cat. 15-4888    Phycoerythrin-Cy5 (PE-Cy5) Armenian Hamster IgG Isotype Control (clone eBio299Arm)
Cat. 11-5811    FITC anti-mouse gamma delta TCR (gd-TCR, gdTCR, TCRgd) (clone UC7-13D5)
Cat. 13-5811    Biotin anti-mouse gamma delta TCR (gd-TCR, gdTCR, TCRgd) (clone UC7-13D5)
Cat. 14-5811    Affinity Purified anti-mouse gamma delta TCR (gd-TCR, gdTCR, TCRgd) (clone UC7-13D5)
Cat. 16-5811    Functional Grade Purified anti-mouse gamma delta TCR (gd-TCR, gdTCR, TCRgd) (clone UC7-13D5)
Cat. 36-5811    Functional Grade Biotin anti-mouse gamma delta TCR (gd-TCR, gdTCR, TCRgd) (clone UC7-13D5)

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

1.         Sale, lease, license or other transfer of the material or any material derived or produced from it.
2.         Sale, lease, license or other grant of rights to use this material or any other material derived or produced from it
3.         Use of this material to perform services for a fee for third parties.

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