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Anti-Mouse gamma delta TCR PE-Cy5

Also known as: gd TCR, gdTCR, TCRgd

RUO: For Research Use Only
mouse gamma delta TCR antibody, Phycoerythrin-Cy5 (PE-Cy5) conjugatemouse gamma delta TCR antibody, Phycoerythrin-Cy5 (PE-Cy5) conjugateStaining of C57Bl/6 lymph node cells with Anti-Mouse CD3e PE (cat. 12-0031) and 0.5 µg of Armenian Hamster IgG Isotype Control PE-Cy5 (cat. 15-4888) (left) or 0.5 µg of Anti-Mouse gamma delta TCR PE-Cy5 (right). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Mouse gamma delta TCR PE-Cy5
Catalog Number: 15-5711
Concentration: 0.2 mg/ml
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material.
Clone: eBioGL3 (GL-3, GL3)
Host/Isotype: Armenian Hamster IgG
 
Product Options
Cat. No. Size Price Add Qty to Cart
15-5711-81 50 ug
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15-5711-82 100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
 
Research Related Products
Cat. No.Format
11-5811 Anti-Mouse gamma delta TCR FITC (UC7-13D5)
13-5811 Anti-Mouse gamma delta TCR Biotin (UC7-13D5)
14-5811 Anti-Mouse gamma delta TCR Purified (UC7-13D5)
15-4888 Armenian Hamster IgG Isotype Control PE-Cy5 (eBio299Arm)
16-5811 Anti-Mouse gamma delta TCR Functional Grade Purified (UC7-13D5)
 

Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
11-5711 Anti-Mouse gamma delta TCR FITC 488 518 FC RUO
12-5711 Anti-Mouse gamma delta TCR PE 488 575 FC RUO
13-5711 Anti-Mouse gamma delta TCR Biotin N/A N/A FC RUO
14-5711 Anti-Mouse gamma delta TCR Purified N/A N/A FC IP RUO
15-5711 Anti-Mouse gamma delta TCR PE-Cy5 488 670 FC RUO
17-5711 Anti-Mouse gamma delta TCR APC 633 660 FC RUO
46-5711 Anti-Mouse gamma delta TCR PerCP-eFluor® 710 488 710 FC RUO
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
 
Description
The GL3 monoclonal antibody (mAb) reacts with the γδ T-cell Receptor complex (TCR) on all γδ TCR bearing T cells, but it does not react with aß TCR. The γδ TCR is present on cells in the thymus, epidermis, epithelial lining of the intestine, peritoneal cavity, and lymphoid tissues.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This GL3 (GL-3) antibody has been reported for use in flow cytometric analysis.

It is recommended to prestain cells with anti-mouse CD16/32 (cat. 14-0161) to prevent non-specific Fc-mediated binding of the GL3 monoclonal antibody.
Applications Tested
This eBioGL3 (GL-3, GL3) antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Goodman, T., and L. LeFrançois. 1989. Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function. J. Exp. Med. 170: 1569-1581.

MacDonald, H. R., M. Schreyer, R. C. Howe, and C. Bron. 1990. Selective expression of CD8a (Ly-2) subunit on activated thymic γδ cells. Eur. J. Immunol. 20: 927-930.

LeFrançois, L. 1991. Phenotypic complexity of intraepithelial lymphocytes of the small intestine. J. Immunol. 147: 1746-1751.
Legal
FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.

COMMERCIAL USE shall include:
1. sale, lease, license or other transfer of the material or any material derived or produced from it;
2. sale, lease, license or other grant of rights to use this Material or any material derived or produced from it;
2. use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO EBIOSCIENCE, INC. 10255 SCIENCE CENTER DRIVE, SAN DIEGO, CALIFORNIA 92121 USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.

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