PE-Cy5 anti-mouse MHC Class II antibody: Cy5PE conjugate

Print Page

Adobe PDF

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-mouse Mouse MHC Class II
Catalog Number: 15-5322
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: NIMR-4
Isotype: Rat IgG2b

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
15-5322-81	50 ug	$155
15-5322-82	100 ug	$265

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5322	Affinity Purified anti-mouse MHC Class II	N/A	N/A	FA FC
16-5322	Functional Grade* Purified anti-mouse MHC Class II	N/A	N/A	FA FC
13-5322	Biotin anti-mouse MHC Class II	N/A	N/A	FC
11-5322	Fluorescein isothiocyanate (FITC) anti-mouse MHC Class II	488	518	FC
12-5322	Phycoerythrin (PE) anti-mouse MHC Class II	488	575	FC
15-5322	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse MHC Class II	488	670	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody NIMR-4 reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells and certain epithelial cells. MHC Class II molecules are important for the selection of the TCR repertoire during the processes of positive and negative selection. MHC Class II expression is required for the positive selection of CD4+ T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This NIMR-4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This NIMR-4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Mielenz, D., C. Vetterman, M. Hampel, C. Lang, A. Avramidou, M. Karas, and H. Jack. 2005. Lipid rafts associate with intracellular B cell receptors and exhibit a B cell stage-specific protein composition. J. Immunol. 174: 3508-3517. (NIMR-4, FC, FA, PubMed).

Related Products

Cat. 15-4031    Phycoerythrin-Cy5 (PE-Cy5) Rat IgG2b Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

1.         Sale, lease, license or other transfer of the material or any material derived or produced from it.
2.         Sale, lease, license or other grant of rights to use this material or any other material derived or produced from it
3.         Use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO eBioscience AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.