Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD135 (Flk-2/Flt-3, Ly-72)
Catalog Number: 15-1351
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: A2F10
Isotype: Rat IgG2a, κ

Staining of BALB/c bone marrow cells with FITC anti-mouse CD45.1 (A20) (cat. 11-0453) and 0.5 μg of PE-Cy5 anti-mouse CD135 (A2F10). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1351	Affinity Purified anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72)	N/A	N/A	FA FC IP
16-1351	Functional Grade* Purified anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72)	N/A	N/A	FA FC
13-1351	Biotin anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72)	N/A	N/A	FC
12-1351	PE anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72)	488	575	FC
15-1351	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72)	488	670	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This A2F10 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ogawa, M., S. Sugawara, et al. (1998). "Flt3/Flk-2 and c-Kit are not essential for the proliferation of B lymphoid progenitor cells in the bone marrow of the adult mouse." Exp Hematol 26(6): 478-88.

Related Products

Cat. 15-4321    PE-Cy5 Rat IgG2a Isotype Control

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