PE-Cy5 anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72) antibody: Cy5PE conjugate

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD135 (Flk-2/Flt-3, Ly-72)
Catalog Number: 15-1351
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: A2F10
Isotype: Rat IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
15-1351-81	50 ug	$185
15-1351-82	100 ug	$295

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c bone marrow cells with FITC anti-mouse CD45.1 (A20) (cat. 11-0453) and 0.5 μg of PE-Cy5 anti-mouse CD135 (A2F10). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1351	Affinity Purified anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72)	N/A	N/A	FA FC IP
16-1351	Functional Grade* Purified anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72)	N/A	N/A	FA FC
13-1351	Biotin anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72)	N/A	N/A	FC
12-1351	PE anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72)	488	575	FC
15-1351	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD135 (Flk-2, Flk2, Flt3, Flt-3, Ly-72)	488	670	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This A2F10 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ogawa, M., S. Sugawara, et al. (1998). "Flt3/Flk-2 and c-Kit are not essential for the proliferation of B lymphoid progenitor cells in the bone marrow of the adult mouse." Exp Hematol 26(6): 478-88.

Related Products

Cat. 15-4321    PE-Cy5 Rat IgG2a Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

1.         Sale, lease, license or other transfer of the material or any material derived or produced from it.
2.         Sale, lease, license or other grant of rights to use this material or any other material derived or produced from it
3.         Use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO eBioscience AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.