Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-human CD107a (LAMP-1)
Catalog Number: 15-1079
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioH4A3
Isotype: Mouse IgG1, κ

Intracellular staining of Jurkat cells with 0.25 μg of PE-Cy5 Mouse IgG1, K Iso Cntrl (cat. 15-4714) (open histogram) or PE-Cy5 anti-human CD107a (H4A3) (colored histogram). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1079	Affinity Purified anti-human CD107a (LAMP-1)	N/A	N/A	IC Flow IHC WB
13-1079	Biotin anti-human CD107a (LAMP-1)	N/A	N/A	IC Flow
11-1079	Fluorescein isothiocyanate (FITC) anti-human CD107a (LAMP-1)	488	518	IC Flow
12-1079	Phycoerythrin (PE) anti-human CD107a (LAMP-1)	488	575	IC Flow
15-1079	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD107a (LAMP-1)	488	670	IC Flow
51-1079	Alexa Fluor® 647 anti-human CD107a (LAMP-1)	633	668	IC Flow
53-1079	Alexa Fluor® 488 anti-human CD107a (LAMP-1)	488	519	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as LAMP-1 (lysosomal-associated membrane protein 1). CD107a, an approximately 110kDa protein, is highly glycosylated, and along with LAMP-2 (CD107b) is estimated to contribute to approximately 50% of all proteins of the lysosomal membrane. CD107a is expressed intracellularly on many cell types, and on the surface of activated platelets and T cells, as well as some cancer cells. It is reported that surface CD107a binds to CD62E (E-Selectin).

It is recommended to use the brightest fluorochromes (cat. 12-1079) for staining for rather than purified and biotinylated formats. Please see other formats for data comparison.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioH4A3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This eBioH4A3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human PBMCs. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.

References

Sarafian V, Jans R, Poumay Y. Expression of lysosome-associated membrane protein 1 (LAMP-1) and galectins in human keratinocytes is regulated by differentiation. Arch. Dermatol. Res. 2006 Jul; 298(2):73-81. (H4A3, IHC, WB, PubMed)

Huynh C, Andrews NW. The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis. EMBO Rep. 2005 Sep;6(9):843-7. (H4A3, FC, IHC, PubMed)

Sarafian V, Jadot M, Foidart JM, Letesson JJ, Van den Brule F, Castronovo V, Wattiaux R, Coninck SW. Expression of Lamp-1 and Lamp-2 and their interactions with galectin-3 in human tumor cells. Int. J. Cancer. 1998 Jan; 75(1):105-111. (H4A3, FC, IHC, PubMed)

Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986;51:97-112.

Related Products

Cat. 15-4714  Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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