Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-human CD95 (Fas/APO-1)
Catalog Number: 15-0959
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: DX2
Isotype: Mouse IgG1, κ
HLDA No.: VI C64

Surface staining of normal human peripheral blood cells with anti-human CD95 (DX2) FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0959	Affinity Purified anti-human CD95 (Fas/APO-1)	N/A	N/A	FA FC IHC
13-0959	Biotin anti-human CD95 (Fas/APO-1)	N/A	N/A	FC
11-0959	FITC anti-human CD95 (Fas/APO-1)	488	518	FC
12-0959	PE anti-human CD95 (Fas/APO-1)	488	575	FC
15-0959	PE-Cy5 anti-human CD95 (Fas/APO-1)	488	670	FC
17-0959	Allophycocyanin (APC) anti-human CD95 (Fas/APO-1)	633	660	FC
25-0959	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD95 (Fas/APO-1)	488	760	FC
57-0959	Pacific Blue® anti-human CD95 (Fas/APO-1)	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. DX2 does not block binding of EOS9.1, another antibody specific for human CD95.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DX2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The DX2 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.

Related Products

Cat. 14-0958    Affinity Purified anti-human CD95 (Fas/APO-1) (clone EOS9.1)
Cat. 16-0958    Functional Grade Purified anti-human CD95 (Fas/APO-1) (clone EOS9.1)
Cat. 15-4714    Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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