PE-Cy5 anti-human CD95 (Fas, APO-1) antibody: Cy5PE conjugate

Print Page

Adobe PDF

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-human CD95 (Fas/APO-1)
Catalog Number: 15-0959
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: DX2
Isotype: Mouse IgG1, κ
HLDA No.: VI C64

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
15-0959-71	25 tests	$120
15-0959-73	100 tests	$260

*International customers: Please contact your distributor for region specific pricing.

Surface staining of normal human peripheral blood cells with anti-human CD95 (DX2) FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0959	FITC anti-human CD95 (Fas/APO-1)	488	518	FC
12-0959	PE anti-human CD95 (Fas/APO-1)	488	575	FC
13-0959	Biotin anti-human CD95 (Fas/APO-1)	N/A	N/A	FC
14-0959	Affinity Purified anti-human CD95 (Fas/APO-1)	N/A	N/A	FA FC IHC
15-0959	PE-Cy5 anti-human CD95 (Fas/APO-1)	488	670	FC
17-0959	Allophycocyanin (APC) anti-human CD95 (Fas/APO-1)	633	660	FC
57-0959	Pacific Blue® anti-human CD95 (Fas/APO-1)	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. DX2 does not block binding of EOS9.1, another antibody specific for human CD95.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DX2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The DX2 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.

Related Products

Cat. 14-0958    Affinity Purified anti-human CD95 (Fas/APO-1) (clone EOS9.1)
Cat. 16-0958    Functional Grade Purified anti-human CD95 (Fas/APO-1) (clone EOS9.1)
Cat. 15-4714    Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

1.         Sale, lease, license or other transfer of the material or any material derived or produced from it.
2.         Sale, lease, license or other grant of rights to use this material or any other material derived or produced from it
3.         Use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO eBioscience AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.