Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD90.2 (Thy-1.2, Thy1.2)
Catalog Number: 15-0902
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 53-2.1
Isotype: Rat IgG2a, κ

Staining of BALB/c splenocytes with staining buffer (autofluorescence)(open histogram) or 0.03 µg PE-Cy5 anti-mouse CD90.2 (53-2.1) (colored histogram). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0902	FITC anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	518	FC
12-0902	PE anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	575	FC
13-0902	Biotin anti-mouse CD90.2 (Thy-1.2, Thy1.2)	N/A	N/A	FC
14-0902	Affinity Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2)	N/A	N/A	FC IH/F IP
15-0902	PE-Cy5 anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	670	FC
17-0902	APC anti-mouse CD90.2 (Thy-1.2, Thy1.2)	633	660	FC
25-0902	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD90.2 (Thy-1.2, Thy1.2)	488	760	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 53-2.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.

Related Products

Cat. 11-0903    FITC anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 30-H12)
Cat. 12-0903    PE anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 30-H12)
Cat. 13-0903    Biotin anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 30-H12)
Cat. 14-0903    Affinity Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 30-H12)
Cat. 16-0903    Functional Grade Purified anti-mouse CD90.2 (Thy-1.2, Thy1.2) (clone 30-H12)
Cat. 15-4321    PE-Cy5 Rat IgG2a Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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