PE-Cy5 anti-human CD86 (B7-2, B72) antibody: Cy5PE conjugate

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Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-human CD86 (B7-2)
Catalog Number: 15-0869
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: IT2.2
Isotype: Mouse IgG2b, κ
HLDA No.: VI B-CD86.8

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
15-0869-71	25 tests	$120
15-0869-73	100 tests	$260

*International customers: Please contact your distributor for region specific pricing.

Surface staining of normal human peripheral blood cells with anti-human CD86 (IT2.2) PE. Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0869	PE anti-human CD86 (B7-2, B72)	488	575	FC
13-0869	Biotin anti-human CD86 (B7-2, B72)	N/A	N/A	FC
14-0869	Affinity Purified anti-human CD86 (B7-2, B72)	N/A	N/A	FA FC
15-0869	PE-Cy5 anti-human CD86 (B7-2, B72)	488	670	FC
16-0869	Functional Grade* Purified anti-human CD86 (B7-2, B72)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The IT2.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This IT2.2 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Kishimoto T, von dem Borne AEG, et al. eds. Leucocyte Typing VI: White Cell Differentiation Antigens. 1998. Garland Publishing Inc. London.

Azuma M, Ito D, Yagita H, Okumura K, Phillips JH, Lanier LL, Somoza C. B70 antigen is a second ligand for CTLA-4 and CD28. Nature. 1993 Nov 4;366(6450):76-9. (IT2.2, FC, IP, PubMed)

Kapsogeorgou EK, Moutsopoulos HM, Manoussakis MN. Functional expression of a costimulatory B7.2 (CD86) protein on human salivary gland epithelial cells that interacts with the CD28 receptor, but has reduced binding to CTLA4. J Immunol. 2001 Mar 1;166(5):3107-13. (IT2.2, FA, PubMed)

Jeannin P, Delneste Y, Lecoanet-Henchoz S, Gauchat JF, Ellis J, Bonnefoy JY. CD86 (B7-2) on human B cells. A functional role in proliferation and selective differentiation into IgE- and IgG4-producing cells. J Biol Chem. 1997 Jun 20;272(25):15613-9. (IT2.2, FA, PubMed)

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Cat. 15-4732    Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG2b Isotype Control

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