Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD86 (B7-2)
Catalog Number: 15-0862
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: GL1
Isotype: Rat IgG2a, κ

Staining of 3-day LPS activated BALB/c splenocytes with 0.03 μg of PE-Cy5 Rat IgG2a Iso Cntrl (cat. 15-4321) (open histogram) or PE-Cy5 anti-mouse CD86 (GL1) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0862	Affinity Purified anti-mouse CD86 (B7-2, B72)	N/A	N/A	FA FC IH/F IP
16-0862	Functional Grade* Purified anti-mouse CD86 (B7-2, B72)	N/A	N/A	FA FC
13-0862	Biotin anti-mouse CD86 (B7-2, B72)	N/A	N/A	FC
11-0862	FITC anti-mouse CD86 (B7-2, B72)	488	518	FC
12-0862	PE anti-mouse CD86 (B7-2, B72)	488	575	FC
15-0862	PE-Cy5 anti-mouse CD86 (B7-2, B72)	488	670	FC
17-0862	APC anti-mouse CD86 (B7-2, B72)	633	660	FC
93-0862	eFluor^TM 605^NC anti-mouse CD86 (B7-2, B72)	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 & CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The GL1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The GL1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocyte suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hathcock, K. S., G. Laszlo, et al. (1993). "Identification of an alternative CTLA-4 ligand costimulatory for T cell activation [see comments]." Science 262(5135): 905-7.
Freeman, G. J., F. Borriello, et al. (1993). "Murine B7-2, an alternative CTLA4 counter-receptor that costimulates T cell proliferation and interleukin 2 production." J Exp Med 178(6): 2185-92.
Inaba, K., M. Witmer-Pack, et al. (1994). "The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro." J Exp Med 180(5): 1849-60.
Hathcock, K. S., G. Laszlo, et al. (1994). "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." J Exp Med 180(2): 631-40

Related Products

Cat. 12-0861    PE anti-mouse CD86 (B7-2, B72) (clone PO3.1)
Cat. 14-0861    Affinity Purified anti-mouse CD86 (B7-2, B72) (clone PO3.1)
Cat. 16-0861    Functional Grade Purified anti-mouse CD86 (B7-2, B72) (clone PO3.1)
Cat. 15-4321    PE-Cy5 Rat IgG2a Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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