Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-human CD45RO
Catalog Number: 15-0457
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: UCHL1
Isotype: Mouse IgG2a, κ
HLDA No.: IV N31

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or PE-Cy5 UCHL1 (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0457	Affinity Purified anti-human CD45RO	N/A	N/A	FC IHC
11-0457	FITC anti-human CD45RO	488	518	FC
12-0457	PE anti-human CD45RO	488	575	FC
15-0457	PE-Cy5 anti-human CD45RO	488	670	FC
17-0457	Allophycocyanin (APC) anti-human CD45RO	633	660	FC
95-0457	eFluor^TM 650^NC anti-human CD45RO	350-500	650	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The UCHL1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The UCHL1 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.03 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 15-4724  Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG2a, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

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