Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)
Catalog Number: 15-0451
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 30-F11
Host/Isotype: Rat IgG2b, κ

mouse CD45 antibody, Phycoerythrin-Cy5 (PE-Cy5) conjugate

Staining of C57Bl/6 splenocytes with 0.03 μg of PE-Cy5 Rat IgG2b Iso Cntrl (cat. 15-4331) (open histogram) or 0.03 μg of PE-Cy5 anti-mouse CD45 (30-F11) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0451	FITC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	518	FC
12-0451	PE anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	575	FC
13-0451	Biotin anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	N/A	N/A	FC
14-0451	Affinity Purified anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	N/A	N/A	FA FC IHC IP
15-0451	PE-Cy5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	670	FC
16-0451	Functional Grade* Purified anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	N/A	N/A	FA FC IHC IP
17-0451	APC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	633	660	FC
25-0451	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	760	FC
35-0451	PE-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	488	690	FC
45-0451	PerCP-Cy5.5 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (PE-Cy5.5 replacement)	488	690	FC
47-0451	Allophycocyanin-eFluor® 780 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (APC-Alexa Fluor® 750 Replacement)	633	780	FC
48-0451	eFluor® 450 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (Pacific Blue® replacement)	405	450	FC
56-0451	Alexa Fluor® 700 anti-mouse CD45 (Leukocyte Common Antigen, Ly-5) (APC-Cy5.5 Equivalent)	633	723	FC
93-0451	eFluor® 605NC anti-mouse CD45 (Leukocyte Common Antigen, Ly-5)	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 30-F11 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.

Related Products

Cat. 15-4031 Phycoerythrin-Cy5 (PE-Cy5) Rat IgG2b Isotype Control

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