Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-human CD33
Catalog Number: 15-0338
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: WM-53 (WM53)
Isotype: Mouse IgG1, κ
HLDA No.: VI M505

Surface staining of normal human peripheral blood cells with anti-human CD33 (WM-53) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0338	FITC anti-human CD33	488	518	FC
12-0338	PE anti-human CD33	488	575	FC
14-0338	Affinity Purified anti-human CD33	N/A	N/A	FC IHC IP MIC
15-0338	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD33	488	670	FC
25-0338	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD33	488	760	FC
45-0338	PerCP-Cy5.5 anti-human CD33	488	690	FC
56-0338	Alexa Fluor® 700 anti-human CD33 (APC-Cy5.5 Equivalent)	633	723	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The WM-53 (WM53) antibody has been reported for use in flow cytometric analysis.

Applications Tested

The WM-53 (WM53) antibody has been pre-titrated and tested by flow cytometric analysis of lysed whole blood. This can be used at 20 μl (0.25 μg) per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC. 1987 Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33). Dis Markers. 5(4):215-25.

Related Products

Cat. 11-0339    FITC anti-human CD33 (clone HIM3-4)
Cat. 12-0339    PE anti-human CD33 (clone HIM3-4)
Cat. 14-0339    Affinity Purified anti-human CD33 (clone HIM3-4)
Cat. 15-0339    PE-Cy5 anti-human CD33 (clone HIM3-4)
Cat. 15-4714    Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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