Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-human CD21 (CR2)
Catalog Number: 15-0217
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BU32 (BU-32)
Isotype: Mouse IgG1
HLDA No.: IV

Surface staining of normal human peripheral blood cells with anti-human CD21 (BU32) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0217	Phycoerythrin (PE) anti-human CD21 (CR2)	488	575	FC
15-0217	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD21 (CR2)	488	670	FC

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Description

The BU32 monoclonal antibody reacts with human CD21, an approximately 145 kDa type I transmembrane protein. CD21 is expressed by mature B cells, folliculate dendritic cells and a subset of epithelial cells and is a receptor for complement component C3d and Epstein-Barr virus (EBV). CD21, in association with CD19 and CD81, participates in the multimolecular complex with BCR and is involved in B cell activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The BU32 (BU-32) antibody has been reported for use in flow cytometric analysis.

Applications Tested

The BU32 (BU-32) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl (0.15 μg) per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 15-4714    Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG1, K Isotype Control

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