Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD11c (Integrin α_X, p150/90)

RUO: For Research Use Only

mouse CD11c antibody, Phycoerythrin-Cy5 (PE-Cy5) conjugate

Staining of BALB/c splenocytes with FITC anti-mouse/human B220 (RA3-6B2) (cat. 11-0452) and 0.25 μg of PE-Cy5 Golden Syrian Hamster IgG Iso Cntrl (cat. 15-4914) (left) or 0.25 μg PE-Cy5 anti-mouse CD11c (N418) (right). Total cells were used for analysis.

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD11c (Integrin α_X, p150/90)
Catalog Number: 15-0114
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Clone: N418
Host/Isotype: Armenian Hamster IgG
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
15-0114-81	50 ug	To view relevant pricing, please select your country and resume browsing.
15-0114-82	100 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
15-4888	Phycoerythrin-Cy5 (PE-Cy5) Armenian Hamster IgG Isotype Control (eBio299Arm)

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-0114	FITC anti-mouse CD11c (Integrin aX, p150/90)	488	518	FC	RUO
12-0114	PE anti-mouse CD11c (Integrin aX, p150/90)	488	575	FC	RUO
13-0114	Biotin anti-mouse CD11c (Integrin aX, p150/90)	N/A	N/A	FC	RUO
14-0114	Affinity Purified anti-mouse CD11c (Integrin aX, p150/90)	N/A	N/A	FC IH/F IP	RUO
15-0114	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD11c (Integrin aX, p150/90)	488	670	FC	RUO
16-0114	Functional Grade* Purified anti-mouse CD11c (Integrin aX, p150/90)	N/A	N/A	FC	RUO
17-0114	APC anti-mouse CD11c (Integrin aX, p150/90)	633	660	FC	RUO
25-0114	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD11c (Integrin aX, p150/90)	488	760	FC	RUO
35-0114	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD11c (Integrin aX, p150/90)	488	690	FC	RUO
45-0114	PerCP-Cy5.5 anti-mouse CD11c (Integrin aX, p150/90) (PE-Cy5.5 replacement)	488	690	FC	RUO
47-0114	Allophycocyanin-eFluor® 780 anti-mouse CD11c (Integrin aX, p150/90) (APC-Alexa Fluor® 750 replacement)	633	780	FC	RUO
48-0114	eFluor® 450 anti-mouse CD11c (Integrin aX, p150/90) (Pacific Blue® Replacement)	405	450	FC	RUO
51-0114	Alexa Fluor® 647 anti-mouse CD11c (Integrin aX, p150/90)	633	668	FC	RUO
53-0114	Alexa Fluor® 488 anti-mouse CD11c (Integrin aX, p150/90)	488	519	FC	RUO
56-0114	Alexa Fluor® 700 anti-mouse CD11c (Integrin aX, p150/90) (APC-Cy5.5 Equivalent)	633	723	FC	RUO
94-0114	eFluor® 625NC anti-mouse CD11c (Integrin aX, p150/90)	350-500	625	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The N418 monoclonal antibody reacts with mouse CD11c, the integrin α_X. CD11c non-covalently associates with β₂ integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The N418 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The N418 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Xin KQ, Mizukami H, Urabe M, Toda Y, Shinoda K, Yoshida A, Oomura K, Kojima Y, Ichino M, Klinman D, Ozawa K, Okuda K. Induction of robust immune responses against human immunodeficiency virus is supported by the inherent tropism of adeno-associated virus type 5 for dendritic cells. J Virol. 2006 Dec;80(24):11899-910. (N418, FC, PubMed)

Ohteki T, Tada H, Ishida K, Sato T, Maki C, Yamada T, Hamuro J, Koyasu S. Essential roles of DC-derived IL-15 as a mediator of inflammatory responses in vivo. J Exp Med. 2006 Oct 2;203(10):2329-38. (N418, IHC frozen, PubMed)

Zhang J, Kawashima N, Suda H, Nakano Y, Takano Y, Azuma M. The existence of CD11c+ sentinel and F4/80+ interstitial dendritic cells in dental pulp and their dynamics and functional properties. Int Immunol. 2006 Sep;18(9):1375-84. (N418, IHC frozen, PubMed)

Guiducci C, Vicari AP, Sangaletti S, Trinchieri G, Colombo MP. Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection. Cancer Res. 2005 Apr 15;65(8):3437-46. (N418, IHC frozen, PubMed)

Esche C, Gambotto A, Satoh Y, Gerein V, Robbins PD, Watkins SC, Lotze MT, Shurin MR. CD154 inhibits tumor-induced apoptosis in dendritic cells and tumor growth.Eur J Immunol. 1999 Jul;29(7):2148-55.

Finkelman FD, Lees A, Birnbaum R, Gause WC, Morris SC. Dendritic cells can present antigen in vivo in a tolerogenic or immunogenic fashion.J Immunol. 1996 Aug 15;157(4):1406-14.

Crowley MT, Inaba K, Witmer-Pack MD, Gezelter S, Steinman RM. Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen. J Immunol Methods. 1990 Oct 4;133(1):55-66.

Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, Steinman RM. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J Exp Med. 1990 May 1;171(5):1753-71.

Legal

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.

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Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD11c (Integrin αX, p150/90)

Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD11c (Integrin α_X, p150/90)