15-0082

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Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD8 (beta subunit; CD8β, Ly-3)
Catalog Number: 15-0082
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CT-CD8b
Isotype: Rat IgG2a

Surface staining of mouse splenocytes with 0.5μg PE-Cy5 (left) and APC (right) anti-mouse CD8b (CT-CD8b). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0082	Affinity Purified Rat anti-mouse CD8 (beta subunit; CD8b, Ly-3)	N/A	N/A	FC
13-0082	Biotin Rat anti-mouse CD8 (beta subunit; CD8b, Ly-3)	N/A	N/A	FC
12-0082	Phycoerythrin (PE) Rat anti-mouse CD8 (beta subunit; CD8b, Ly-3)	488	575	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The CT-CD8b monoclonal antibody reacts with the mouse CD8β molecule. The CD8β chain associates with the CD8α chain to form the CD8α/β heterodimer expressed on the surface of a majority of thymocytes and a subpopulation of mature αβ TCR T cells. However, mature γδ TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 αα homodimers. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CT-CD8b antibody has been reported for use in flow cytometric analysis.

Applications Tested

The CT-CD8b antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1.0 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.
Ledbetter, J. A., R. V. Rouse, et al. (1980). "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." J Exp Med 152(2): 280-95.

Related Products

Cat. 15-4321    PE-Cy5 Rat IgG2a Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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