Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-human CD7
Catalog Number: 15-0077
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CD7-6B7
Isotype: Mouse IgG2a
HLDA No.: IV

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or PE-Cy5 anti-human CD7 (CD7-6B7) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Description

The CD7-6B7 monoclonal antibody reacts with human CD7, a 40 kDa type I transmembrane protein. CD7 is expressed by thymocytes, mature T cells, NK cells and pre-B cells. Signaling through crosslinking of CD7 on the surface of T cells induces calcium ion flux.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CD7-6B7 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The CD7-6B7 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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