Product Information

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-rat CD5
Catalog Number: 15-0050
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIS47
Isotype: Mouse IgG2a, κ

Staining of rat bone marrow with staining buffer (autofluorescence) (open histogram) or .125 μg of PE-Cy5 anti-rat CD5 (HIS47) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0050	Affinity Purified anti-rat CD5	N/A	N/A	FC IH/F
11-0050	FITC anti-rat CD5	488	518	FC
12-0050	PE anti-rat CD5	488	575	FC
15-0050	Phycoerythrin-Cy5 (PE-Cy5) anti-rat CD5	488	670	FC
17-0050	Allophycocyanin (APC) anti-rat CD5	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIS47 monoclonal antibody reacts with rat CD5, a 67 kDa surface protein expressed by a majority of thymocytes and mature T cells. While mouse and human CD5 is also expressed on a small subset of B cells called B-1 cells, such CD5⁺ B cell subset has not been identified in the rat so far. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HIS47 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HIS47 antibody has been tested by flow cytometric analysis of rat bone marrow and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Vermeer LA, de Boer NK, Bucci C, Bos NA, Kroese FG, Alberti S. 1994. MRC OX19 recognizes the rat CD5 surface glycoprotein, but does not provide evidence for a population of CD5bright B cells. Eur J Immunol. 24(3):585-92.

Vermeer L, Bos N, Kroese F, Bucci C, Alberti S. 1992. Molecular cloning of the rat CD5 gene. Ann N Y Acad Sci. 651:82-3.
de Boer NK, Ammerlaan WA, Meedendorp B, Kroese FG. 1992. CD5 B cells in the rat? Ann N Y Acad Sci. 651:157-9.

Related Products

Cat. 15-4724  Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG2a, K Isotype Control

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