PE-Cy5 anti-human CD2 (LFA-2, LFA2) antibody: Cy5PE conjugate

Contents: Phycoerythrin-Cy5 (PE-Cy5) anti-human CD2 (LFA-2)
Catalog Number: 15-0029
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RPA-2.10
Isotype: Mouse IgG1, κ
HLDA No.: IV T085

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
15-0029-71	25 tests	$120
15-0029-73	100 tests	$260

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1, K Iso Cntrl (cat. 15-4714) (open histogram) or PE-Cy5 anti-human CD2 (RPA-2.10) (colored histogram). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0029	FITC anti-human CD2 (LFA-2)	488	518	FC
12-0029	PE anti-human CD2 (LFA-2)	488	575	FC
13-0029	Biotin anti-human CD2 (LFA-2)	N/A	N/A	FC
14-0029	Affinity Purified anti-human CD2 (LFA-2)	N/A	N/A	FA FC IH/F WB
15-0029	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD2 (LFA-2)	488	670	FC
16-0029	Functional Grade* Purified anti-human CD2 (LFA-2)	N/A	N/A	FC
17-0029	Allophycocyanin (APC) anti-human CD2 (LFA-2)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This RPA-2.10 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This RPA-2.10 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Aversa GG, Bishop GA, Suranyi MG, Hall BM. (1987). RPA-2.10: an anti-CD2 monoclonal antibody that inhibits alloimmune responses and monitors T cell activation. Transplant Proc. 19:277-8

Related Products

Cat. 15-4714    Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

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