Product Information

Contents: Affinity Purified anti-human IgM
Catalog Number: 14-9998
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.1% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: SA-DA4
Isotype: Mouse IgG1, κ

Surface staining of prelysed normal human peripheral blood cells with anti-human IgM (SA-DA4) Purified followed by anti-mouse IgG FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9998	Affinity Purified anti-human IgM	N/A	N/A	ELISA FC IH/F
13-9998	Biotin anti-human IgM	N/A	N/A	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The SA-DA4 monoclonal antibody reacts with the μ heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The SA-DA4 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA. It has also been reported for use in affinity purification of human IgM.

Applications Tested

The SA-DA4 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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