Surface staining of normal human peripheral blood cells with anti-human IgL, λ (JDC-12) Purified followed by anti-mouse IgG Biotin and SA-PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The JDC-12 monoclonal antibody reacts with the λ light chain of human immunoglobulin. It does not react with the κ light chain. Ig light chain is expressed as a membrane-bound form as well as soluble form.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The JDC-12 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA.
Applications Tested
The JDC-12 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
