Staining of normal human peripheral blood cells with Anti-Human Ig lambda Light Chain Purified followed by Anti-Mouse IgG Biotin and Streptavidin PE (cat. 12-4317). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The JDC-12 monoclonal antibody reacts with the λ light chain of human immunoglobulin. It does not react with the κ light chain. Ig light chain is expressed as a membrane-bound form as well as soluble form.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The JDC-12 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA.
Applications Tested
The JDC-12 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
