Product Information

Contents: Affinity Purified anti-human Perforin
Catalog Number: 14-9995
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 1B4
Isotype: Mouse IgG2b, κ

Immunoblotting of human PBMC cell lysate from 10⁵ cells; probed with 1 ug per mL of purified 1B4 (Lane A) and revealed with HRP anti-mouse IgG.

Description

The 1B4 antibody reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granuels of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 1B4 antibody has been reported for use in immunoblotting (WB).

Applications Tested

The 1B4 antibody has been tested by immunoblotting (WB) of perforin from human PBMC lysate at about 1ug/ml. A band at approximate size of 70 kDa could be detected.

References

Ortaldo JR, Winkler-Pickett RT, Nagashima K, Yagita H, Okumura K. 1992. Direct evidence for release of pore-forming protein during NK cellular lysis. J Leukoc Biol. 52(5):483-8.

Uellner R, Zvelebil MJ, Hopkins J, Jones J, MacDougall LK, Morgan BP, Podack E, Waterfield MD, Griffiths GM. 1997. Perforin is activated by a proteolytic cleavage during biosynthesis which reveals a phospholipid-binding C2 domain. EMBO J. 16(24):7287-96.

Nakata M, Kawasaki A, Azuma M, Tsuji K, Matsuda H, Shinkai Y, Yagita H, Okumura K. 1992. Expression of perforin and cytolytic potential of human peripheral blood lymphocyte subpopulations. Int Immunol. 4(9):1049-54.

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