Print Page

Adobe PDF

Contents: Affinity Purified anti-human Perforin Catalog Number: 14-9995 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: 1B4 Isotype: Mouse IgG2b, κ

Immunoblotting of human PBMC cell lysate from 105 cells; probed with 1 ug per mL of purified 1B4 (Lane A) and revealed with HRP anti-mouse IgG.

---

Description

---

The 1B4 antibody reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granuels of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.

---

Applications Reported

---

For research use only, not for diagnostic or therapeutic use. This 1B4 antibody has been reported for use in immunoblotting (WB).

---

Applications Tested

---

The 1B4 antibody has been tested by immunoblotting (WB) of perforin from human PBMC lysate at about 1ug/ml. A band at approximate size of 70 kDa could be detected.

---

References

---

Ortaldo JR, Winkler-Pickett RT, Nagashima K, Yagita H, Okumura K. 1992. Direct evidence for release of pore-forming protein during NK cellular lysis. J Leukoc Biol. 52(5):483-8.

Uellner R, Zvelebil MJ, Hopkins J, Jones J, MacDougall LK, Morgan BP, Podack E, Waterfield MD, Griffiths GM. 1997. Perforin is activated by a proteolytic cleavage during biosynthesis which reveals a phospholipid-binding C2 domain. EMBO J. 16(24):7287-96.

Nakata M, Kawasaki A, Azuma M, Tsuji K, Matsuda H, Shinkai Y, Yagita H, Okumura K. 1992. Expression of perforin and cytolytic potential of human peripheral blood lymphocyte subpopulations. Int Immunol. 4(9):1049-54.

---

Related Products

---

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4732    Affinity Purified Mouse IgG2b Isotype Control
Cat. 18-8877    See Catalog # 18-8817 for an alternative product: Mouse TrueBlot™: Horseradish Peroxidase (HRP) anti-mouse IgG