Product Information

Contents: Affinity Purified anti-human Perforin
Catalog Number: 14-9994
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: dG9 (delta G9)
Isotype: Mouse IgG2b, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9994	Affinity Purified anti-human Perforin	N/A	N/A	IHC IP
11-9994	Fluorescein isothiocyanate (FITC) anti-human Perforin	488	518	IC Flow IHC IP
12-9994	Phycoerythrin (PE) anti-human Perforin	488	575	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. (Fluorochrome conjugated dG9 (delta G9) is recommended for use in intracellular flow cytometry.)

Applications Tested

This dG9 (delta G9) antibody has been tested by intracellular staining and flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

M.J. Smyth, et al, (1990) J Exp Med 171: 1269-1281.
A. Hameed, et al, (1992) Am J Pathol 140: 1025-1030.
P.L. Jose, et al, (1994) J Immunol 148: 3354-3360.
B.C. Schlesinger & L. Cheng, (1994) Immunol 81: 291-295.
C.C. Liu, et al, (1995) Immunol Today 16: 194-201.

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