Product Information

Contents: Affinity Purified anti-human Perforin
Catalog Number: 14-9993
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: eBioBOR21
Isotype: Mouse IgG2b

Immunoblotting of human PBMC (Lane A) or PBMC stimulated with IL-2 for 3 days (Lane B) lysate; probed with 2 µg/mL purified eBioBOR21 and anti-actin and revealed with HRP anti-rat IgG or anti-mouse IgG, respectivley. Peforin is detected at ~72 kDa and actin at ~42 kDa. Actin was used a loading control to normalize the signal

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9993	Affinity Purified anti-human Perforin	N/A	N/A	WB

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Description

The eBioBOR21 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Applications Tested

The eBioBOR21 antibody has been tested for use in immunoblotting (WB) of human PBMCs at 1-5µg/ml. It is recommended that this antibody be titrated for optimal performance in the assay of interest.

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