mouse CXCR4 (Fusin, CD184) antibody: purified antibody

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Contents: Affinity Purified anti-mouse CXCR4 (Fusin, CD184)
Catalog Number: 14-9991
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 2B11
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-9991-80	25 ug	$80
14-9991-82	100 ug	$180

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c Thymocytes with 0.5 μg of Purified Rat IgG2b Isotype Control (cat. 14-4031) (open histogram) or 0.5 μg of Purified anti-mouse CXCR4 (2B11) (colored histogram) followed by Biotin Anti-Rat IgG (cat. 13-4813)and SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9991	Affinity Purified anti-mouse CXCR4 (Fusin, CD184)	N/A	N/A	FC IH/F WB
16-9991	Functional Grade* Purified anti-mouse CXCR4 (Fusin, CD184)	N/A	N/A	FA FC
12-9991	Phycoerythrin (PE) anti-mouse CXCR4 (Fusin, CD184)	488	575	FC
51-9991	Alexa Fluor® 647 anti-mouse CXCR4 (Fusin, CD184)	633	668	FC
53-9991	Alexa Fluor® 488 anti-mouse CXCR4 (Fusin, CD184)	488	519	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 2B11 monoclonal antibody reacts with mouse and human CXCR4, also known as CD184, Fusin, LESTR, or HUMSTR. Fusin is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. In addition it has been shown to play a role in lymphocyte migration. The ligand for CXCR4 is SDF1. CXCR4 is required for maintenance of granulocyte progenitors in the bone marrow and essential for B cell development. Expression of CXCR4 is different between mice and human. In the thymus CXCR4 is restricted to CD4+CD8+ cells while in the spleen predominant expression is found on B lymphocytes.
The monoclonal antibody 2B11 recognizes the amino terminus of CXCR4 and has been reported to have functional activity; it does not block SDF1a but causes internalization of the receptor.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 2B11 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistology staining of frozen tissue sections.

Applications Tested

This 2B11 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Forster R, Kremmer E, Schubel A, Breitfeld D, Kleinschmidt A, Nerl C, Bernhardt G, Lipp M. Intracellular and surface expression of the HIV-1 coreceptor CXCR4/fusin on various leukocyte subsets: rapid internalization and recycling upon activation. J Immunol. 1998 Feb 1;160(3):1522-31. (2B11, FC, WB, FA, PubMed)

Schabath R, Muller G, Schubel A, Kremmer E, Lipp M, Forster R. The murine chemokine receptor CXCR4 is tightly regulated during T cell development and activation. J Leukoc Biol. 1999 Dec;66(6):996-1004.(2B11, FC, WB PubMed)

Stumm RK, Rummel J, Junker V, Culmsee C, Pfeiffer M, Krieglstein J, Hollt V, Schulz S. A dual role for the SDF-1/CXCR4 chemokine receptor system in adult brain: isoform-selective regulation of SDF-1 expression modulates CXCR4-dependent neuronal plasticity and cerebral leukocyte recruitment after focal ischemia. J Neurosci. 2002 Jul 15;22(14):5865-78. (2B11, WB, PubMed)

Related Products

Cat. 14-4031    Purified Rat IgG2b Isotype Control
Cat. 11-4811    FITC Anti-Rat IgG
Cat. 14-9999    Affinity Purified anti-human CXCR4 (Fusin, CD184) (clone 12G5)