human HLA-ABC antibody: purified antibody

Contents: Affinity Purified anti-human HLA-ABC
Catalog Number: 14-9983
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: W6/32
Isotype: Mouse IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-9983-80	25 ug	$50
14-9983-82	100 ug	$95

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with 0.5 μg of Purified Mouse IgG2a, K Iso Cntrl (cat. 14-4724) (open histogram) or 0.5 μg of Purified anti-human HLA-ABC (W6/32) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9983	Affinity Purified anti-human HLA-ABC	N/A	N/A	FC IH/F IP
16-9983	Functional Grade* Purified anti-human HLA-ABC	N/A	N/A	FC
13-9983	Biotin anti-human HLA-ABC	N/A	N/A	FC
11-9983	FITC anti-human HLA-ABC	488	518	FC
12-9983	PE anti-human HLA-ABC	488	575	FC
15-9983	PE-Cy5 anti-human HLA-ABC	488	670	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This W6/32 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.

Applications Tested

The W6/32 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Barnstable, C. J., W. F. Bodmer, et al. 1978. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell 14(1): 9-20.

Koene G, Arts-Hilkes, et al.2004. High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss. Hum Immunol. 65(12):1455-62. (IHC frozen, PubMed)

Goldman-Wohl D, Ariel I, et al. 2001. A study of human leukocyte antigen G expression in hydatidiform moles. Am J Obstet Gynecol. 185(2):476-80. (IHC frozen, PubMed)

Stern PL, Beresford N, et al. 1987. Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast. J Immunol. 138(4):1088-91. (baboon cross-reactivity, PubMed)

Related Products

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4724    Affinity Purified Mouse IgG2a, K Isotype Control