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Human Cellular Antigens Chart

Contents: Affinity Purified anti-human CD45RA Catalog Number: 14-9979 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles. Clone: JS-83 Isotype: Mouse IgG1, κ HLDA No.: N/A Prices for This Product* Cat. No. Size Price Qty Action 14-9979-80 25 ug $50 14-9979-82 100 ug $95 *International customers: Please contact your distributor for region specific pricing.

Surface staining of normal human peripheral blood cells with anti-human CD45RA (JS-83) FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-9979	FITC anti-human CD45RA	488	518	FC
12-9979	PE anti-human CD45RA	488	575	FC
13-9979	Biotin anti-human CD45RA	N/A	N/A	FC
14-9979	Affinity Purified anti-human CD45RA	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The JS-83 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4⁺ peripheral T lymphocytes, CD8⁺ peripheral T lymphocytes, and B cells. The CD45RA⁺ T cell populations are mainly naive/virgin allowing the use of JS-83 mAb as a phenotypic marker to discriminate T cell subsets.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. JS-83 has been reported for use in flow cytometric analysis.

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Applications Tested

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The JS-83 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

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