Product Information

Contents: Affinity Purified anti-human HAI-1 (HGF activator inhibitor-1)
Catalog Number: 14-9960
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 9B10
Isotype: Mouse IgG2a, κ

Staining of MCF7 cell line with 0.25 μg of Purified Mouse IgG2a, K Iso Cntrl (cat. 14-4724) (open histogram) or 0.25 μg of Purified anti-human HAI-1 (HGF activator inhibtor-1) (colored histogram) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (cat. 12-4012). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9960	Affinity Purified anti-human HAI-1 (HGF activator inhibitor-1)	N/A	N/A	FC IP WB
12-9960	Phycoerythrin (PE) anti-human HAI-1 (HGF activator inhibitor-1)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody 9B10 recognizes HAI-1 also known as hepatocyte growth factor activation inhibitor. HAI-1 is a single transmembrane protein of approximately 53 kDa with an extracellular Kunitz-type serine protease inhibitor domain. HAI-1 and HAI-2 are produced in membrane-associated forms, which are secreted as active, proteolytically truncated proteins. HAI can bind to and inhibit HGFA and matripase, which are responsible for converting HGF to an active form. Human HAI-1 transcript is expressed in various human tissues, such as adult placenta, kidney, pancreas, prostate, small intestine, fetal kidney and fetal lung. HAI-1 is a marker for prostate and breast cancers.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 9B10 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB) under nonreducing conditions only.

Applications Tested

This 9B10 antibody has been tested by flow cytometric analysis of human cell line MCF7. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Parr C, Watkins G, Mansel RE, Jiang WG. The hepatocyte growth factor regulatory factors in human breast cancer. Clin Cancer Res. 2004 Jan 1;10(1 Pt 1):202-11.

Oberst M, Anders J, Xie B, Singh B, Ossandon M, Johnson M, Dickson RB, Lin CY. Matriptase and HAI-1 are expressed by normal and malignant epithelial cells in vitro and in vivo. Am J Pathol. 2001 Apr;158(4):1301-11.

Suzuki K, Nakamura K, Kato K, Hamada H, Tsukamoto T. Exploration of target molecules for prostate cancer gene therapy. Prostate. 2007 Aug 1;67(11):1163-73. (9B10, FC, IP, PubMed)

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