Product Information

Contents: Affinity Purified anti-human HLA- MHC Class I free chain without B2 microglobulin
Catalog Number: 14-9958
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: A4
Isotype: Mouse IgG1

Staining of normal human peripheral blood mononuclear cells either unactivated (left) or 3-day PHA-activated (right) with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human HLA- MHC Class I free chain without B2m (A4) (colored histogram) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L)(Minimal Reactivity to Rat IgG) (cat. 12-4012). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9958	Affinity Purified anti-human HLA- MHC Class I free chain without B2 microglobulin	N/A	N/A	FC IHC(Paraffin) IP
51-9958	Alexa Fluor® 647 anti-human HLA- MHC Class I free chain without B2 microglobulin	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The A4 monoclonal antibody reacts with the class I human leukocyte antigen (HLA) complex in the absence of β2 microglobulin. There are three class I α-chain genes in humans, called HLA-A, -B and -C. Normally, HLA class I proteins are associated non-covalently with β2 microglobulin (β2M). The function of the HLA class I complex is to present peptides derived from intracellular pathogens, including viral pathogens, on the cell surface to antigen specific CD8+ cytotoxic T cells. Class I HLA is expressed by the majority of nucleated cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This A4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of paraffin embedded tissue sections.

Applications Tested

This A4 antibody has been tested by flow cytometric analysis of 3-day PHA-activated PBMC. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Lopez de Castro JA, Barbosa JA, Krangel MS, Biro PA, Strominger JL. Structural analysis of the functional sites of class I HLA antigens. Immunol Rev. 1985 Jul;85:149-68.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)