Product Information

Contents: Affinity Purified anti-human HLA-DR
Catalog Number: 14-9956
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: LN3
Isotype: Mouse IgG2b, κ

Staining of normal human peripheral blood cells with 0.125 μg of Purified Mouse IgG2b Iso Cntrl (cat. 14-4732) (open histogram) or 0.125 μg of Purified anti-human HLA-DR (LN3) (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9956	Affinity Purified anti-human HLA-DR	N/A	N/A	FC IHC
13-9956	Biotin anti-human HLA-DR	N/A	N/A	FC
11-9956	Fluorescein isothiocyanate (FITC) anti-human HLA-DR	488	518	FC
12-9956	PE anti-human HLA-DR	488	575	FC
15-9956	Phycoerythrin-Cy5 (PE-Cy5) anti-human HLA-DR	488	670	FC
17-9956	APC anti-human HLA-DR	633	660	FC
25-9956	Phycoerythrin-Cy7 (PE-Cy7) anti-human HLA-DR	488	760	FC
56-9956	Alexa Fluor® 700 anti-human HLA-DR (APC-Cy5.5 Equivalent)	633	723	FC
27-9956	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human HLA-DR	633	779	FC
45-9956	PerCP-Cy5.5 anti-human HLA-DR	488	690	FC
93-9956	eFluor^TM 605^NC anti-human HLA-DR	350-500	605	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of α and β subunits and plays an important role in the presentation of peptides to CD4⁺ T lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The LN3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. Immunohistochemistry can be performed using frozen and Bouin’s, or formalin/paraffin, human tissues.

Applications Tested

The LN3 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Fullen, D. R. and J. T. Headington (1998). "Factor XIIIa-positive dermal dendritic cells and HLA-DR expression in radial versus vertical growth-phase melanomas [see comments]." J Cutan Pathol 25(10): 553-8.
Hua, Z. X., K. E. Tanaka, et al. (1998). "Immunoreactivity for LN2 and LN3 distinguishes small cell carcinomas from non-small cell carcinomas in the lung." Hum Pathol 29(12): 1441-6.
Ioachim, H. L., S. E. Pambuccian, et al. (1996). "Lymphoid monoclonal antibodies reactive with lung tumors. Diagnostic applications." Am J Surg Pathol 20(1): 64-71.
Davey, F. R., S. Olson, et al. (1988). "The immunophenotyping of extramedullary myeloid cell tumors in paraffin-embedded tissue sections." Am J Surg Pathol 12(9): 699-707.
Norton, A. J. and P. G. Isaacson (1987). "Detailed phenotypic analysis of B-cell lymphoma using a panel of antibodies reactive in routinely fixed wax-embedded tissue." Am J Pathol 128(2): 225-40.

Related Products

Cat. 11-4011    FITC Anti-Mouse IgG
Cat. 13-4013    Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4732    Affinity Purified Mouse IgG2b Isotype Control