Surface staining of CD11.3 cell line with Anti-Mouse H-Y TCR (male antigen) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The T3.70 monoclonal antibody reacts with the transgenic αβTCR that recognizes the male antigen H-Y in the context of H-2Db. A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor. The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic major histocompatibility complex in CD4+ and CD8+ T cell differentiation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The T3.70 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The T3.70 antibody has been tested by flow cytometric analysis of CD11.3 line, which is derived from a thymic tumor of the H-Y TCR transgenic mice and expresses the transgenic H-Y TCR. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Teh, H. S., H. Kishi, et al. (1990). "Early deletion and late positive selection of T cells expressing a male-specific receptor in T-cell receptor transgenic mice." Dev Immunol 1(1): 1-10. Teh, H. S., H. Kishi, et al. (1989). "Deletion of autospecific T cells in T cell receptor (TCR) transgenic mice spares cells with normal TCR levels and low levels of CD8 molecules." J Exp Med 169(3): 795-806. Teh, H. S., P. Kisielow, et al. (1988). "Thymic major histocompatibility complex antigens and the alpha beta T-cell receptor determine the CD4/CD8 phenotype of T cells." Nature 335(6187): 229-33.
