Product Information

Contents: Affinity Purified anti-human MD-2 (MD2; Toll-like Receptor/ TLR Family)
Catalog Number: 14-9928
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 9B4
Isotype: Mouse IgG1

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9928	Purified anti-human MD-2 (MD2; Toll-like Receptor/ TLR Family)	N/A	N/A	IP

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Description

The 9B4 antibody reacts with human MD-2, an approximately 30 kDa molecule, which physically associates with the extracellular domain of TLR4. MD-2 is essential for correct intracellular distribution and LPS-recognition of TLR4 since in MD-2(-/-) embryonic fibroblasts, TLR4 was not able to reach the plasma membrane and predominantly resided in the Golgi apparatus, whereas TLR4 was distributed at the leading edge surface of cells in wild-type embryonic fibroblasts. MD-2(-/-) mice do not respond to LPS, do survive endotoxic shock but are susceptible to Salmonella typhimurium infection.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 9B4 antibody has been reported for use in immunoprecipitation.

Applications Tested

This 9B4 antibody has been tested by immunoprecipitation of recombinant MD-2 from cell supernatant. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Thompson PA, Tobias PS, Viriyakosol S, Kirkland TN, Kitchens RL. 2003. Lipopolysaccharide (LPS)-binding protein inhibits responses to cell-bound LPS. J Biol Chem. 278(31):28367-71.
Viriyakosol S, Tobias PS, Kitchens RL, Kirkland TN. 2001. MD-2 binds to bacterial lipopolysaccharide. J Biol Chem. 276(41):38044-51.

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