Product Information

Contents: Rabbit anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4)
Catalog Number: 14-9920
Sizes: 50 ug, 200 ug
Formulation: This product is supplied as purified rabbit IgG fraction in PBS containing 0.02% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
The 50µg size of this antibody is supplied in liquid form. The 200µg size of this antibody may be supplied in liquid or lyophilized powder forms. To reconstitute the lyophilized antibody, add 200µl of PBS for a final concentration of 1mg/ml.
Clone: Polyclonal
Isotype: Rabbit IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9920	Affinity Purified anti-human Toll-like receptor 4 (CD284, TLR4, TLR-4) Polyclonal	N/A	N/A	WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

This polyclonal antibody, which reacts with the TLR4 protein, was generated using E. coli-expressed human TLR4 (a.a. 6-169) as an immunogen. So far, at least ten members of the Toll family have been identified in humans. This family of type I transmembrane protein is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4 is expressed by peripheral blood monocytes. Cross-reactivity of this polyclonal with other TLRs has not been determined.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in immunoblotting (WB). The tested titer for immunoblotting is 1:3,000. This polyclonal has been reported to detect human TLR4 (~100 kDa) from transfected cells. Cross-reactivity with other TLRs has not been determined.

Applications Tested

Purified anti-human TLR4 poly has been tested by immunoblotting of lysate from human TLR4 transfected cells.

References

Rock, F.L., et al. (1998) A family of human receptors structurally related to Drosophila Toll. PNAS 95:588-93.
Medzhitov, R. et al. (1997) A human homologue of the Drosophila Toll protein signals activation of adaptive immunity. Nature 388:394-7.
Poltorak, A. et al. (1998) Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science 282:2085-8.

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