Product Information

Contents: Affinity Purified anti-human EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1)
Catalog Number: 14-9326
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 1B7
Isotype: Mouse IgG1, κ

Staining of A549 human lung carcinoma cells with 0.125 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.125 μg of Purified anti-human EpCAM (1B7) (colored histogram) followed by PE Donkey Anti-Mouse IgG (cat. 12-4012). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9326	Affinity Purified anti-human EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1)	N/A	N/A	FC IHC(Paraffin) IP WB
12-9326	Phycoerythrin (PE) anti-human EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1)	488	575	FC
51-9326	Alexa Fluor® 647 anti-human EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1)	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kD cell-surface adhesion molecule participating in homophilic, calcium-independent cell-cell interactions. EpCAM is a type-I transmembrane protein, and is expressed primarily on the basolateral surface of most epithelia. Although normal epithelia express low levels of EpCAM, increased expression has been correlated with increased proliferation and progression to a mesenchymal phenotype. EpCAM has also been used as a diagnostic marker on circulating metastatic carcinoma cells, while cancer cells of non-epithelial origin do not express EpCAM.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 1B7 antibody has been reported for use in flow cytometric analysis, immunohistochemistry on paraffin-embedded sections, and immunoprecipitation. The 1B7 antibody has also been found useful for immunoblotting, and recognizes a protein of approximately 40 kD under non-reducing conditions.

Applications Tested

This 1B7 antibody has been tested by flow cytometric analysis of A549 human lung carcinoma cells. This can be used at less than or equal to 0.25 µg per 100 µl blood (or per 1 million cells in 100 µl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Suzuki K, Nakamura K, Kato K, Hamada H, Tsukamoto T. Exploration of target molecules for prostate cancer gene therapy. Prostate. 2007 Aug 1;67(11):1163-73. (1B7, FC, IHC, PubMed)

Rao CG, Chianese D, Doyle GV, Miller MC, Russell T, Sanders RA Jr, Terstappen LW. Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors. Int J Oncol. 2005 Jul;27(1):49-57. (PubMed)

Litvinov SV, Bakker HA, Gourevitch MM, Velders MP, Warnaar SO. Evidence for a role of the epithelial glycoprotein 40 (Ep-CAM) in epithelial cell-cell adhesion. Cell Adhes Commun. 1994 Oct;2(5):417-28. (PubMed)

Litvinov SV, Velders MP, Bakker HA, Fleuren GJ, Warnaar SO. Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule. J Cell Biol. 1994 Apr;125(2):437-46. (PubMed)

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