Product Information

Contents: Affinity Purified anti-human CD321 (F11R, JAM-1, JAM-A)
Catalog Number: 14-9321
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: WK9
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with 0.125 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.125 μg of Purified anti-human CD321 (WK9) (colored histogram) followed by PE Donkey Anti-Mouse IgG (cat. 12-4012). Cells in the lymphocyte (left) and monocyte (right) gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9321	Affinity Purified anti-human CD321 (F11R, JAM-1, JAM-A)	N/A	N/A	FC IP WB
51-9321	Alexa Fluor® 647 anti-human CD321 (F11R, JAM-1, JAM-A)	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors. CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail. The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins. CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, epithelial and endothelial cells, and plays a role in mediating cell-cell contact. CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin. CD321 is also involved in reovirus entry into cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This WK9 antibody has been reported for use in flow cytometric analysis and immunoprecipitation. The WK9 antibody has also been found useful for immunoblotting, recognizing a protein of approximately 38 kD under reducing and non-reducing conditions.

Applications Tested

This WK9 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 µg per 100 µl blood (or per 1 million cells in 100 µl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sobocka MB, Sobocki T, Banerjee P, Weiss C, Rushbrook JI, Norin AJ, Hartwig J, Salifu MO, Markell MS, Babinska A, Ehrlich YH, Kornecki E. Cloning of the human platelet F11 receptor: a cell adhesion molecule member of the immunoglobulin superfamily involved in platelet aggregation. Blood. 2000 Apr 15;95(8):2600-9.(PubMed)

Ostermann G, Weber KS, Zernecke A, Schroder A, Weber C. JAM-1 is a ligand of the beta(2) integrin LFA-1 involved in transendothelial migration of leukocytes. Nat Immunol. 2002 Feb;3(2):151-8.(PubMed)

Naik MU, Mousa SA, Parkos CA, Naik UP. Signaling through JAM-1 and alphavbeta3 is required for the angiogenic action of bFGF: dissociation of the JAM-1 and alphavbeta3 complex. Blood. 2003 Sep 15;102(6):2108-14. (PubMed)

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