Product Information

Contents: Affinity Purified anti-human CD230 (Prion protein)
Catalog Number: 14-9230
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 4D5
Isotype: Mouse IgG1, κ

Staining of normal human peripheral blood cells with 0.25 μg of Purified Mouse IgG1, K Iso Cntrl (cat. 14-4714) (open histogram) or 0.25 μg of Purified anti-human CD230 (4D5) (colored histogram) followed by PE Donkey Anti-Mouse IgG (cat. 12-4012). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9230	Affinity Purified anti-human CD230 (Prion protein)	N/A	N/A	FC IP WB
12-9230	Phycoerythrin (PE) anti-human CD230 (Prion protein)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

CD230 (prion protein; PrP^C) is a 35 kD glycoprotein, attached to the cell surface via a GPI-anchor. While the function of CD230 is unclear, prion protein has been the focus of research based on its involvement in transmissible spongiform encephalopathies (TSE), including Creutzfeld-Jakob disease in humans. CD230 undergoes a conformational change in which the cellular PrP^C form is converted to the scrapie PrP^Sc form. This conversion is essential for the infectiousness of prion-based diseases. CD230 is predominantly expressed on the surface of lymphocytes and monocytes, with weak expression on granulocytes and erythrocytes. In platelets, CD230 is expressed primarily intracellularly, and is upregulated to the surface upon activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 4D5 antibody has been reported for use in flow cytometric analysis and immunoprecipitation. The 4D5 antibody has also been found useful for immunoblotting, recognizing a protein of approximately 35 kD under non-reducing conditions.

Applications Tested

The 4D5 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.5 µg per 100 µl blood (or per 1 million cells in 100 µl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Durig J, Giese A, Schulz-Schaeffer W, Rosenthal C, Schmucker U, Bieschke J, Duhrsen U, Kretzschmar HA. Differential constitutive and activation-dependent expression of prion protein in human peripheral blood leucocytes. Br J Haematol. 2000 Mar;108(3):488-95. (PubMed)

Barclay GR, Hope J, Birkett CR, Turner ML. Distribution of cell-associated prion protein in normal adult blood determined by flow cytometry. Br J Haematol. 1999 Dec;107(4):804-14. (PubMed)

Holada K, Simak J, Risitano AM, Maciejewski J, Young NS, Vostal JG. Activated platelets of patients with paroxysmal nocturnal hemoglobinuria express cellular prion protein. Blood. 2002 Jul 1;100(1):341-3. (PubMed)

Related Products

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Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4714  Affinity Purified Mouse IgG1, K Isotype Control