Contents: Affinity Purified Human FcγR-Binding Inhibitor
Catalog Number: 14-9161
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
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U937 cells were stained with FITC mouse IgG2a (left plot) (cat. 11-4724) or FITC mouse IgG2b (right plot) (cat. 11-4732) isotype controls without (purple shading) or with (blue line) pre-treatment with affinity purified Human FcγR-Binding Inhibitor. The red line represents autofluorescence of U937 cells.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-9161 |
Affinity Purified Human FcgR-Binding Inhibitor |
N/A |
N/A |
FC
|
| 16-9161 |
Functional Grade* Purified Human FcgR-Binding Inhibitor |
N/A |
N/A |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The human FcγR-binding inhibitor can be used to inhibit the non-specific Fc-gamma receptor (FcγR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of human tissue. Four different classes of Fcγ receptors have been identified: FcγRI (CD64), FcγRII (CD32), FcγRIII (CD16), and FcγRIV, and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcγR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcγR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcγRs.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Purified FcγR-binding inhibitor has been reported for use in flow cytometric analysis.
To inhibit the non-specific binding of mouse monoclonal antibodies add the human FcγR-binding inhibitor to sample and incubate on ice for 20 minutes. Without washing proceed to stain with primary antibody.
Applications Tested
Purified human FcγR-binding inhibitor has been pre-titrated and tested by inhibiting binding of fluorochrome-conjugated isotype controls to U937 cells. This can be used at 20 μl per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
References
Nimmerjahn F, Ravetch JV.
Fcgamma receptors: old friends and new family members.
Immunity. 2006 Jan;24(1):19-28. Review.
Gessner JE, Heiken H, Tamm A, Schmidt RE.
The IgG Fc receptor family.
Ann Hematol. 1998 Jun;76(6):231-48. Review.
Human Cellular Antigens Chart