human FcR antibody: purified antibody

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Contents: Affinity Purified Human FcγR-Binding Inhibitor
Catalog Number: 14-9161
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
14-9161-71	25 tests	$75
14-9161-73	100 tests	$140

*International customers: Please contact your distributor for region specific pricing.

U937 cells were stained with FITC mouse IgG2a (left plot) (cat. 11-4724) or FITC mouse IgG2b (right plot) (cat. 11-4732) isotype controls without (purple shading) or with (blue line) pre-treatment with affinity purified Human FcγR-Binding Inhibitor. The red line represents autofluorescence of U937 cells.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9161	Affinity Purified Human FcgR-Binding Inhibitor	N/A	N/A	FC
16-9161	Functional Grade* Purified Human FcgR-Binding Inhibitor	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The human FcγR-binding inhibitor can be used to inhibit the non-specific Fc-gamma receptor (FcγR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of human tissue. Four different classes of Fcγ receptors have been identified: FcγRI (CD64), FcγRII (CD32), FcγRIII (CD16), and FcγRIV, and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcγR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcγR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcγRs.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Purified FcγR-binding inhibitor has been reported for use in flow cytometric analysis.

To inhibit the non-specific binding of mouse monoclonal antibodies add the human FcγR-binding inhibitor to sample and incubate on ice for 20 minutes. Without washing proceed to stain with primary antibody.

Applications Tested

Purified human FcγR-binding inhibitor has been pre-titrated and tested by inhibiting binding of fluorochrome-conjugated isotype controls to U937 cells. This can be used at 20 μl per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Nimmerjahn F, Ravetch JV. Fcgamma receptors: old friends and new family members. Immunity. 2006 Jan;24(1):19-28. Review.

Gessner JE, Heiken H, Tamm A, Schmidt RE. The IgG Fc receptor family. Ann Hematol. 1998 Jun;76(6):231-48. Review.