Product Information

Contents: Affinity Purified anti-human BR3 (CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)
Catalog Number: 14-9117
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Avoid repeated freeze/thaw cycles.
Clone: 8A7
Isotype: Mouse IgG2a, κ

Staining of normal human peripheral blood cells with Purified Mouse IgG2a, κ Iso Cntrl (cat. 14-4724) (open histogram) or Purified 8A7 (colored histogram) followed by FITC Anti-Mouse IgG (cat. 11-4011). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9117	Purified anti-human BR3 (CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)	N/A	N/A	FC IHC WB
16-9117	Functional Grade* Purified anti-human BR3 (CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)	N/A	N/A	FA FC
13-9117	Biotin anti-human BR3 (CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)	N/A	N/A	FC
11-9117	Fluorescein isothiocyanate (FITC) anti-human BR3 (CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)	488	518	FC
12-9117	Phycoerythrin (PE) anti-human BR3 (CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 8A7 monoclonal antibody reacts with the human BAFF receptor, also known as B lymphocyte stimulator (BLyS protein) receptor, which is expressed on B cells. The ligand for this receptor, BAFF (B cell-activating factor of the TNF family) is a B cell survival factor and regulates CD21/35 and CD23 expression. Interaction of this ligand with its receptor causes elevated CD21/35 and CD23 expression, whereas receptor blockade has been shown to down-modulate expression.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 8A7 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. It has also been reported for use in functional assays. (Please use Functional Grade purified 8A7, cat. 16-9117, in functional assays.)

Applications Tested

The 8A7 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nakamura, N., H. Hase, D. Sakurai, S. Yoshida, M. Abe, N. Tsukada, J. Takizawa, S. Aoki, M. Kojima, S. Nakamura, and Tetsuji Kobata. 2005. Expression of BAFF-R (BR3) in normal and neoplastic lymphoid tissues characterized with a newly developed monoclonal antibody, Virchows Archiv. 447: 53 - 60 [Cites use of 8A7 in IHC, flow cytometry, and Western blotting.]

Sakurai D, Kanno Y, Hase H, Kojima H, Okumura K, Kobata T. TACI attenuates antibody production costimulated by BAFF-R and CD40. Eur J Immunol. 2007 Jan;37(1):110-8. PubMed

Related Products

Cat. 11-4011  FITC Anti-Mouse IgG
Cat. 13-4013  Biotin Anti-Mouse IgG (clone Polyclonal)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 14-4724  Affinity Purified Mouse IgG2a, K Isotype Control
Cat. 14-6144  Anti-human, mouse, rat BR3 (CD268, BLyS receptor 3, BAFF-R, BAFF Receptor) polyclonal (clone Polyclonal)
Cat. 18-8877  See Catalog # 18-8817 for an alternative product: Mouse TrueBlot™: Horseradish Peroxidase (HRP) anti-mouse IgG